MYL3 Polyclonal antibody

MYL3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 10913-1-AP

Synonyms

Ventricular myosin alkali light chain, Myosin light chain 3, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MYL 3, MLC-lV/sb

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Validation Data Gallery

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  WB analysis of mouse heart using 10913-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10913-1-AP (MYL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10913-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10913-1-AP (MYL3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 10913-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10913-1-AP (MYL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10913-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10913-1-AP (MYL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 10913-1-AP

  IP result of anti-MYL3 (IP:10913-1-AP, 3ug; Detection:10913-1-AP 1:7000) with mouse heart tissue lysate 4000ug.

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  IHC staining of human heart using 10913-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10913-1-AP (MYL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10913-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10913-1-AP (MYL3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10913-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10913-1-AP (MYL3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C2C12 using 10913-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using MYL3 antibody (10913-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of C2C12 using 10913-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYL3 (10913-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue, human heart tissue, rat heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human heart tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: C2C12 cells
• Positive FC (Intra) detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10913-1-AP targets MYL3 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYL3 fusion protein Ag1364
• Full Name: myosin, light chain 3, alkali; ventricular, skeletal, slow
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22-27 kDa
• GenBank Accession Number: BC009790
• Gene Symbol: MYL3
• Gene ID (NCBI): 4634
• RRID: AB_2147607
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)

Publications

Species	Application	Title
human	WB	Nat Biotechnol; A pipeline that integrates the discovery and verification of plasma protein biomarkers reveals candidate markers for cardiovascular disease.; Authors - Addona Terri A TA
	WB	J Proteome Res; Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy; Authors - Yuan Lin
mouse,human	WB,IHC,IF	Nat Commun; MYL3 protects chondrocytes from senescence by inhibiting clathrin-mediated endocytosis and activating of Notch signaling; Authors - He Cao
mouse	WB	Nat Commun; Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis; Authors - Yesheng Fu

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C