rat heart tissue were subjected to SDS PAGE followed by western blot with 55462-1-AP (MYL2 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
rat heart tissue were subjected to SDS PAGE followed by western blot with 55462-1-AP (MYL2 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 55462-1-AP
IP result of anti-Myosin Light Chain 2/MLC-2V (IP:55462-1-AP, 4ug; Detection:55462-1-AP 1:500) with mouse brain tissue lysate 4000ug.
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55462-1-AP (MYL2 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skeletal muscle using 55462-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55462-1-AP (MYL2 Antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55462-1-AP (MYL2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 55462-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55462-1-AP (MYL2 Antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55462-1-AP (MYL2 Antibody) at dilution of 1:50 (under 40x lens).
FC experiment of C2C12 using 55462-1-AP
1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Myosin Light Chain 2/MLC-2V (55462-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Myosin Light Chain 2/MLC-2V (55462-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected in
C2C12 cells
Positive FC detected in
C2C12 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:20000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)
FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.
Protocols
Product Specific Protocols
WB protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 55462-1-AP (MYL2 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human heart using 55462-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55462-1-AP (MYL2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 55462-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55462-1-AP (MYL2 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skeletal muscle using 55462-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55462-1-AP (MYL2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 55462-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55462-1-AP (MYL2 Antibody) at dilution of 1:50 (under 40x lens).
IP Figures
IP experiment of mouse brain using 55462-1-AP
IP result of anti-Myosin Light Chain 2/MLC-2V (IP:55462-1-AP, 4ug; Detection:55462-1-AP 1:500) with mouse brain tissue lysate 4000ug.
FC (INTRA) Figures
FC experiment of C2C12 using 55462-1-AP
1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Myosin Light Chain 2/MLC-2V (55462-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Myosin Light Chain 2/MLC-2V Polyclonal antibody
Catalog Number
55462-1-AP
Citations
3
Dilutions
WB : 1:2000-1:20000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IP, IHC, ELISA, FC (Intra)
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet