MYL12A Polyclonal antibody

MYL12A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16287-1-AP

Synonyms

MLC 2B, MLCB, MRLC3, MYL12A, MYL2B, Myosin RLC, RLC

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 16287-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16287-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 16287-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse bladder using 16287-1-AP

  mouse bladder tissue were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16287-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16287-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16287-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16287-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 16287-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16287-1-AP (MYL12A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16287-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16287-1-AP (MYL12A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 16287-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16287-1-AP (MYL12A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 16287-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16287-1-AP (MYL12A Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, human skeletal muscle tissue, mouse bladder tissue, Jurkat cells, HEK-293 cells, HeLa cells, mouse heart tissue
• Positive IHC detected in: human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

16287-1-AP targets MYL12A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYL12A fusion protein Ag9413
• Full Name: myosin, light chain 12A, regulatory, non-sarcomeric
• Calculated Molecular Weight: 171 aa, 20 kDa
• Observed Molecular Weight: 19-20 kDa
• GenBank Accession Number: BC016372
• Gene Symbol: MYL12A
• Gene ID (NCBI): 10627
• RRID: AB_2878238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
pig	WB	Biochem J; Kinase-related protein/telokin inhibits Ca2+-independent contraction in Triton-skinned guinea pig taenia coli.; Authors - Shcherbakova Olga V OV
mouse	WB	Front Physiol; Distinct Roles of Smooth Muscle and Non-muscle Myosin Light Chain-Mediated Smooth Muscle Contraction.; Authors - Jie Sun
human	IHC	Biofizika; [Novel peptide inhibitors of the myosin light chain kinase suppress hyperpermeability of vascular endothelium].; Authors - Marchenko A V AV
mouse	WB	EMBO J; Salmonella manipulates macrophage migration via SteC-mediated myosin light chain activation to penetrate the gut-vascular barrier; Authors - Yuanji Dai