MYL1 Polyclonal antibody

MYL1 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15814-1-AP

Synonyms

MLC1/MLC3, MLC1F, MLC1F/MLC3F, MLC3F, MYL1, Myosin light chain A1/A2, Myosin light chain alkali 1/2

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Validation Data Gallery

• 

  WB analysis of human skeletal muscle using 15814-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15814-1-AP (MYL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 15814-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15814-1-AP (MYL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15814-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15814-1-AP (MYL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15814-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15814-1-AP (MYL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 15814-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 15814-1-AP (MYL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human skeletal muscle tissue, human heart tissue, mouse heart tissue, mouse skeletal muscle tissue, mouse skin tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below

Product Information

15814-1-AP targets MYL1 in WB,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYL1 fusion protein Ag8594
• Full Name: myosin, light chain 1, alkali; skeletal, fast
• Calculated molecular weight: 150aa,17 kDa; 194aa,22 kDa
• Observed molecular weight: 14-17 kDa, 22-25 kDa
• GenBank accession number: BC005318
• Gene symbol: MYL1
• Gene ID (NCBI): 4632
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	WB	Nature; SAR1B senses leucine levels to regulate mTORC1 signalling.; Authors - Jie Chen
mouse	WB	Cell Prolif; KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells.; Authors - Jing Hao