MYL1 Polyclonal antibody

MYL1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15814-1-AP

Synonyms

MLC1/MLC3, MLC1F, MLC1F/MLC3F, MLC3F, MYL1, Myosin light chain A1/A2, Myosin light chain alkali 1/2



Tested Applications

Positive WB detected inhuman skeletal muscle tissue, human heart tissue, mouse heart tissue, mouse skeletal muscle tissue, mouse skin tissue
Positive IHC detected inrat skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15814-1-AP targets MYL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYL1 fusion protein Ag8594
Full Name myosin, light chain 1, alkali; skeletal, fast
Calculated Molecular Weight150aa,17 kDa; 194aa,22 kDa
Observed Molecular Weight 14-17 kDa, 22-25 kDa
GenBank Accession NumberBC005318
Gene Symbol MYL1
Gene ID (NCBI) 4632
RRIDAB_2266747
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MYL1 antibody 15814-1-APDownload protocol
IHC protocol for MYL1 antibody 15814-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nature

SAR1B senses leucine levels to regulate mTORC1 signalling.

Authors - Jie Chen
mouseWB

Cell Prolif

KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells.

Authors - Jing Hao