MYCBPAP Polyclonal antibody

MYCBPAP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12763-1-AP

Synonyms

AMAP-1, AMAP1, AMAP 1, AMAM-1, AMAM1

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Validation Data Gallery

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  WB analysis of human placenta using 12763-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12763-1-AP (MYCBPAP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 12763-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12763-1-AP (MYCBPAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12763-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12763-1-AP (MYCBPAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12763-1-AP

  Immunofluorescent analysis of HepG2 cells, using MYCBPAP antibody 12763-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HUVEC using 12763-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using MYCBPAP antibody (12763-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HUVEC cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12763-1-AP targets MYCBPAP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYCBPAP fusion protein Ag3419
• Full Name: MYCBP associated protein
• Calculated Molecular Weight: 947 aa, 108 kDa
• Observed Molecular Weight: 108 kDa
• GenBank Accession Number: BC028393
• Gene Symbol: MYCBPAP
• Gene ID (NCBI): 84073
• RRID: AB_2235719
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYCBPAP, or MYCBP associated protein, is a protein encoded by the human MYCBPAP gene. It is primarily expressed in the testis and is involved in spermatogenesis, the process of forming sperm cells. The protein may also play a role in synaptic processes, which are essential for neuronal signaling. MYCBPAP is predicted to be an intracellular protein with molecular functions including clathrin binding, phospholipid binding, and protein binding. It is associated with biological processes such as cell differentiation, chemical synaptic transmission, endocytosis, and spermatogenesis.

Publications

Species	Application	Title
mouse	WB	Sci China Life Sci; Homozygous deleterious variants in MYCBPAP induce asthenoteratozoospermia involving abnormal acrosome biogenesis, manchette structure and sperm tail assembly in humans and mice; Authors - Yiling Zhou