MYCBP2 Polyclonal antibody

MYCBP2 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 27951-1-AP

Synonyms

Protein associated with Myc, Pam/highwire/rpm 1 protein, PAM, MYC binding protein 2, KIAA0916

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Validation Data Gallery

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  IHC staining of human brain using 27951-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 27951-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 27951-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 27951-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27951-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27951-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 27951-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MYCBP2 antibody (27951-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of U2OS using 27951-1-AP

  1x10^6 U2OS cells were intracellularly stained with 0.4 ug MYCBP2 Polyclonal antibody (27951-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IHC detected in: human brain tissue, rat brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below

Product Information

27951-1-AP targets MYCBP2 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYCBP2 fusion protein Ag27228
• Full Name: MYC binding protein 2
• Calculated Molecular Weight: 510 kDa
• GenBank Accession Number: NM_015057
• Gene Symbol: MYCBP2
• Gene ID (NCBI): 23077
• RRID: AB_2881020
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Front Immunol; MYCBP2 expression correlated with inflammatory cell infiltration and prognosis immunotherapy in thyroid cancer patients; Authors - Guilin Wang
human	IHC	Arch Biochem Biophys; Exosomes from human umbilical cord mesenchymal stem cells protect aortas in Db/db mice characterized by combination of metabolomics and proteomics; Authors - Ming-Ying Ling