"MYC tag Antibodies" Comparison
View side-by-side comparison of MYC tag antibodies from other vendors to find the one that best suits your research needs.
|Positive WB detected in||Recombinant protein protein, Recombinant protein|
|Positive IP detected in||Transfected HEK-293 cells|
|Positive IF detected in||Transfected HEK-293 cells, MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
16286-1-AP targets MYC tag in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
|Tested Reactivity||recombinant protein|
|Cited Reactivity||human, mouse, chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MYC tag fusion protein Ag9409|
|Full Name||MYC tag|
|Gene ID (NCBI)|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The myc-tag is a short synthetic polypeptide sequence derived from c-myc protein that can be added to recombinant proteins to enable isolation and study when an antibody is not available. This antibody recognizes the MYC tag EQKLISEEDL.
What is the molecular weight of myc?
1203Da: the ten amino acid myc tag sequence is EQKLISEEDL (in single letter code).
What are the applications for myc tag?
The addition of the myc tag to a particular protein can be useful when an antibody is not available to the protein of interest. Using recombinant DNA technology, the myc tag can be fused to the protein and then an antibody against the myc tag can be used to probe (PMID: 24490106). This is a reliable method and can be used in a number of different techniques, including purification using chromatography, tracking the protein in localization studies using immunofluorescence, or quantifying levels using Western Blot (PMID: 24490106).
What is the structure of myc tag?
The c-myc gene from which this tag is derived has a molecular weight of 49kDa, but the myc tag represents only a small portion of the C-terminus of this gene. The short polypeptide sequence can be fused to the N-terminus or the C-terminus of any protein without influencing function, although it is advised to avoid fusing it to a secretory signal. A cleavage site behind this tag is also sometimes engineered to allow removal with a specific protease.
Signal Transduct Target Ther
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Ephrin receptor A2 is an epithelial cell receptor for Epstein-Barr virus entry.
TRIM5α recruits HDAC1 to p50 and Sp1 and promotes H3K9 deacetylation at the HIV-1 LTR
Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells.
Nat Struct Mol Biol
ARF GTPases activate Salmonella effector SopF to ADP-ribosylate host V-ATPase and inhibit endomembrane damage-induced autophagy.
CHEK1 and circCHEK1_246aa evoke chromosomal instability and induce bone lesion formation in multiple myeloma.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Amy (Verified Customer) (03-05-2023)
Detected overexpressed Mac-tagged proteins in HEK293T cell lysates but with a fair few background bands.
James (Verified Customer) (11-30-2022)
Very good antibody
Boyan (Verified Customer) (08-03-2022)
gives a good signal in IF
Chahat (Verified Customer) (06-08-2022)
The myc antibody gives clear staining!
WEI (Verified Customer) (03-08-2022)
Good antibody for detecting overexpression proteins in 293 cells.
Minze (Verified Customer) (01-11-2022)
it works well for immunofluorecence so far.
Phoebe (Verified Customer) (03-01-2019)
This antibody was very clean in a western blot to detect Myc-tagged pol2 in S cerevisiae whole cell extract and chromatin fractionation samples.