MYBPC2 Polyclonal antibody

MYBPC2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25257-1-AP

Synonyms

MYBPCF, Fast MyBP C

---

Validation Data Gallery

• 

  WB analysis using 25257-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25257-1-AP (MYBPC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 25257-1-AP

  IP result of anti-MYBPC2 (IP:25257-1-AP, 4ug; Detection:25257-1-AP 1:40000) with mouse skeletal muscle tissue lysate 1040 ug.

---

Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IP detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25257-1-AP targets MYBPC2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYBPC2 fusion protein Ag19424
• Full Name: myosin binding protein C, fast type
• Calculated Molecular Weight: 1141 aa, 128 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC136389
• Gene Symbol: MYBPC2
• Gene ID (NCBI): 4606
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYBPC2, also known as MYBPCF, fMyBP-C, is a member of the myosin-binding protein C (MYBPC)family. MYBPC is a myosin-associated protein found in the cross-bridge-bearing zone, or C region, of A bands in striated muscle. MYBPC has three paralogs encoded by unique genes, including slow skeletal (MYBPC1), fast skeletal (MYBPC2), and cardiac (MYBPC3). Slow skeletal MyBP-C (sMyBP-C), fast skeletal MyBP-C (fMyBP-C), and cardiac MyBP-C (cMyBP-C) proteins are highly conserved with over 90% homology. They play unique muscle-specific structural and regulatory roles in actomyosin interactions and contractility in striated muscles, including both cardiac and skeletal muscles (PMID: 35832193, 23936073).