MVD Polyclonal antibody

MVD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 15331-1-AP

Synonyms

MPD, Mevalonate, EC:4.1.1.33, diphospho

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Validation Data Gallery

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  WB analysis using 15331-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15331-1-AP (MVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 15331-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15331-1-AP (MVD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 15331-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15331-1-AP (MVD Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 15331-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15331-1-AP (MVD Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 15331-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15331-1-AP (MVD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 15331-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15331-1-AP (MVD Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15331-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15331-1-AP (MVD Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 15331-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A431 cells using 15331-1-AP (MVD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HCT 116 cells, rat liver tissue, HepG2 cells, K-562 cells
• Positive IHC detected in: human colon tissue, human lung cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

15331-1-AP targets MVD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MVD fusion protein Ag7255
• Full Name: mevalonate (diphospho) decarboxylase
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 66-74 kDa, 45 kDa, 37 kDa
• GenBank Accession Number: BC000011
• Gene Symbol: MVD
• Gene ID (NCBI): 4597
• RRID: AB_2147439
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The enzyme mevalonate pyrophosphate decarboxylase(MVD) catalyzes the conversion of mevalonate pyrophosphate into isopentenyl pyrophosphate. It is also named as MPD and as a unique enzyme in one of the early steps in cholesterol biosynthesis, MVD may be a useful target for drugs aimed at lowering serum cholesterol levels(PMID:8626466). The intracellular glycosylation does not contribute to the difference between the 45 and 37 kDa species of MVD. The native MVD has a molecular weight of 90 kDa that it consists of two identical subunits of 45 kDa and a 37 kDa protein is also found as a subunit of MVD and this type of MVD may be a 74 kDa.But the 37 kDa enzyme appeared only when the rats are fed the CP diet.(PMID:9348097).

Publications

Species	Application	Title
human	WB	J Hepatocell Carcinoma; miR-612 Enhances RSL3-Induced Ferroptosis of Hepatocellular Carcinoma Cells via Mevalonate Pathway; Authors - Kailin Xing
mouse	IHC	Cell Signal; Circ_0011058 facilitates proliferation, angiogenesis and radioresistance in papillary thyroid cancer cells by positively regulating YAP1 via acting as miR-335-5p sponge.; Authors - Zhenhua Zhang
mouse	WB	Curr Issues Mol Biol; Gigantol Improves Cholesterol Metabolism and Progesterone Biosynthesis in MA-10 Leydig Cells.; Authors - Audrey Basque
human	WB	Front Pharmacol; CCR9 overexpression promotes T-ALL progression by enhancing cholesterol biosynthesis; Authors - Muhammad Jamal

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

k (Verified Customer) (06-10-2021)

We always got good bands in western with this Ab

• Applications: Western Blot
• Primary Antibody Dilution: 1;1000