MUC2 Polyclonal antibody

MUC2 Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

72

Reviews (1)

Cat no : 27675-1-AP

Synonyms

SMUC, Mucin 2, MUC-2, MUC 2, MLP

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Validation Data Gallery

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  IHC staining of human colon using 27675-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 27675-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 27675-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat small intestine using 27675-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 27675-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 27675-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 27675-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat small intestine using 27675-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using MUC2 antibody (27675-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), smooth muscle actin specific antibody (67735-1-Ig, Clone: 1E9A11, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat small intestine using 27675-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using MUC2 antibody (27675-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), smooth muscle actin specific antibody (67735-1-Ig, Clone: 1E9A11, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat small intestine using 27675-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using MUC2 antibody (27675-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004), smooth muscle actin specific antibody (67735-1-Ig, Clone: 1E9A11, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon using 27675-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using MUC2 antibody (27675-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 27675-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using MUC2 antibody (27675-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 27675-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using MUC2 antibody (27675-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SW480 using 27675-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed SW480 cells using MUC2 antibody (27675-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human colon tissue, human pancreas cancer tissue, mouse small intestine tissue, rat small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human colon tissue, mouse colon tissue, rat small intestine tissue
• Positive IF/ICC detected in: HepG2 cells, SW480 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 15 publications below
• IHC: See 29 publications below
• IF: See 34 publications below

Product Information

27675-1-AP targets MUC2 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, duck, mosquito
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MUC2 fusion protein Ag25800
• Full Name: mucin 2, oligomeric mucus/gel-forming
• Calculated Molecular Weight: 540 kDa
• GenBank Accession Number: M94132
• Gene Symbol: MUC2
• Gene ID (NCBI): 4583
• RRID: AB_2880943
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. Downregulation of this gene has been observed in patients with Crohn disease and ulcerative colitis.

Publications

Species	Application	Title
mouse	IF	Nat Commun; Dubosiella newyorkensis modulates immune tolerance in colitis via the L-lysine-activated AhR-IDO1-Kyn pathway; Authors - Yanan Zhang
mouse	IF	EMBO J; Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.; Authors - Rongpan Bai
mouse	IF	Pharmacol Res; λ-carrageenan exacerbates Citrobacter rodentium-induced infectious colitis in mice by targeting gut microbiota and intestinal barrier integrity; Authors - Wei Wu
mouse	IF	NPJ Biofilms Microbiomes; Impact of structurally diverse polysaccharides on colonic mucin O-glycosylation and gut microbiota; Authors - Tong Zhao
human	IHC	Chemosphere; A possible but unrecognized risk of acceptable daily intake dose triazole pesticides exposure-bile acid disturbance induced pharmacokinetic changes of oral medication; Authors - Yujing Xu
mouse	IF	Gut Microbes; Microbial hydrogen economy alleviates colitis by reprogramming colonocyte metabolism and reinforcing intestinal barrier.; Authors - Li Ge

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Balawant (Verified Customer) (08-24-2022)

This is great Antibody. it has no background

• Applications: Western Blot
• Primary Antibody Dilution: 1:1500
• Cell Tissue Type: colon tissue and colon cancer cells line