MTRF1L Polyclonal antibody

MTRF1L Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16694-1-AP

Synonyms

MRF1L, mtRF1a, MTRF1L

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 16694-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16694-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16694-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16694-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16694-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16694-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16694-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

16694-1-AP targets MTRF1L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTRF1L fusion protein Ag10081
• Full Name: mitochondrial translational release factor 1-like
• Calculated Molecular Weight: 380 aa, 44 kDa
• Observed Molecular Weight: 40-46 kDa
• GenBank Accession Number: BC011873
• Gene Symbol: MTRF1L
• Gene ID (NCBI): 54516
• RRID: AB_2878301
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTRF1L, also named as Peptide chain release factor 1-like, mitochondrial, is a 380 amino acid protein, which belongs to the prokaryotic/mitochondrial release factor family and locates in the mitochondrial. MTRF1L directs the termination of translation in response to the peptide chain termination codons UAA and UAG. MTRF1L is expressed in skeletal muscle.

Publications

Species	Application	Title
mouse	WB	Biochem Biophys Res Commun; Muscle denervation reduces mitochondrial biogenesis and mitochondrial translation factor expression in mice.; Authors - Takumi Yokokawa
mouse	WB	Physiol Rep; Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle.; Authors - Takumi Yokokawa
rat	WB	Sci Total Environ; Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects; Authors - Dongjie Li
rat	WB	J Hazard Mater; ZLN005 alleviates PBDE-47 induced impairment of mitochondrial translation and neurotoxicity through PGC-1α/ERRα axis; Authors - Zhiyuan Tian