MTMR2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MTMR2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 14372-1-AP

Synonyms

CMT4B, CMT4B1, KIAA1073, MTMR2, myotubularin related protein 2

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Validation Data Gallery

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  WB analysis of HeLa using 14372-1-AP

  WB result of MTMR2 antibody (14372-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTMR2 transfected HeLa cells.

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  WB analysis using 14372-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14372-1-AP (MTMR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human cerebellum using 14372-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 14372-1-AP (MTMR2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human cerebellum using 14372-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 14372-1-AP (MTMR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse cerebellum using 14372-1-AP

  IP result of anti-MTMR2 (IP:14372-1-AP, 4ug; Detection:14372-1-AP 1:300) with mouse cerebellum tissue lysate 4000 ug.

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  IHC staining of human endometrial cancer using 14372-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14372-1-AP (MTMR2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 14372-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14372-1-AP (MTMR2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cerebellum using 14372-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14372-1-AP (MTMR2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human cerebellum using 14372-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14372-1-AP (MTMR2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, human cerebellum tissue, NIH/3T3 cells
• Positive IP detected in: mouse cerebellum tissue
• Positive IHC detected in: human endometrial cancer tissue, human cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14372-1-AP targets MTMR2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTMR2 fusion protein Ag5745
• Full Name: myotubularin related protein 2
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 66-73 kDa
• GenBank Accession Number: BC052990
• Gene Symbol: MTMR2
• Gene ID (NCBI): 8898
• RRID: AB_2282336
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTMR2(myotubularin-related protein 2) is also named as KIAA1073 and belongs to the non-receptor class myotubularin subfamily, which is characterized by the presence of a phosphatase domain. It may regulate vesicular trafficking in Schwann cells (PMID:17880751) and its reversible phosphorylation represents a critical mechanism for regulating the subcellular localization of MTMR2(PMID:21372139). MTMR2 is a 73 kDa protein in its monomeric form and exist as a dimer within cells and the protein re-localizes to membranes in hypo-osmotic settings(PMID:16399794). Defects in MTMR2 are the cause of Charcot-Marie-Tooth disease type 4B1 (CMT4B1). MTMR2 has two isofroms with the molecular mass of 73 and 66 kDa.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kincaid (Verified Customer) (05-21-2024)

This product worked as expected. I completed a GFP-nanotrap IP (Chromotek agarose beads) and ran 2% whole cell lysate with IP fraction on an SDS-PAGE gel. Resolved proteins were transferred to a PVDF membrane and probed with aMTMR2. GFP-CCDC22 is known to pull-down MTMR2 and is validated with this antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

MTMR2 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:14372-1-AP)