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MTMR2 Polyclonal antibody

MTMR2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14372-1-AP

Synonyms

CMT4B, CMT4B1, KIAA1073, MTMR2, myotubularin related protein 2



Tested Applications

Positive WB detected inHeLa cells, human cerebellum tissue, NIH/3T3 cells
Positive IP detected inmouse cerebellum tissue
Positive IHC detected inhuman endometrial cancer tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14372-1-AP targets MTMR2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTMR2 fusion protein Ag5745
Full Name myotubularin related protein 2
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 66-73 kDa
GenBank Accession NumberBC052990
Gene Symbol MTMR2
Gene ID (NCBI) 8898
RRIDAB_2282336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTMR2(myotubularin-related protein 2) is also named as KIAA1073 and belongs to the non-receptor class myotubularin subfamily, which is characterized by the presence of a phosphatase domain. It may regulate vesicular trafficking in Schwann cells (PMID:17880751) and its reversible phosphorylation represents a critical mechanism for regulating the subcellular localization of MTMR2(PMID:21372139). MTMR2 is a 73 kDa protein in its monomeric form and exist as a dimer within cells and the protein re-localizes to membranes in hypo-osmotic settings(PMID:16399794). Defects in MTMR2 are the cause of Charcot-Marie-Tooth disease type 4B1 (CMT4B1). MTMR2 has two isofroms with the molecular mass of 73 and 66 kDa.

Protocols

Product Specific Protocols
WB protocol for MTMR2 antibody 14372-1-APDownload protocol
IHC protocol for MTMR2 antibody 14372-1-APDownload protocol
IP protocol for MTMR2 antibody 14372-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Kincaid (Verified Customer) (05-21-2024)

This product worked as expected. I completed a GFP-nanotrap IP (Chromotek agarose beads) and ran 2% whole cell lysate with IP fraction on an SDS-PAGE gel. Resolved proteins were transferred to a PVDF membrane and probed with aMTMR2. GFP-CCDC22 is known to pull-down MTMR2 and is validated with this antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
MTMR2 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:14372-1-AP)