MTCO2 Monoclonal antibody

• 

  WB analysis of 143B.TK.P0 using 68527-1-Ig

  WB result of MTCO2 antibody (MTCO2; 1:1000; incubated at room temperature for 1.5 hours) with 143B and 143B.TK.ρ0 cell. 143B is wild-type cell line, 143B.TK.ρ0 is mtDNA deletion cell line.

• 

  WB analysis using 68527-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68527-1-Ig (MTCO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

MTCO2 Monoclonal Antibody for WB, ELISA

Cat No. 68527-1-Ig

Clone No.10G14F4

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

COX2, 10G14F4, COII, COX 2, COXII

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Tested Applications

• Positive WB detected in: U2OS cells, 143B cells, A431 cells, HeLa cells, HepG2 cells, Jurkat cells, LNCaP cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

68527-1-Ig targets MTCO2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: cytochrome c oxidase II
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 23-26 kDa
• GenBank Accession Number: YP_003024029
• Gene Symbol: MTCO2
• Gene ID (NCBI): 4513
• RRID: AB_3085235
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P00403
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Transl Med; DMC-BH derivative DMC-GF inhibits the growth of glioma stem cells by targeting the TRIM33/SLC25A1/mitochondrial oxidative phosphorylation pathway; Authors - Lei Shi