Moesin Polyclonal antibody

• Featured Product
• KD/KO Validated

Moesin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 16495-1-AP

Synonyms

MSN, Membrane-organizing extension spike protein

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Validation Data Gallery

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  WB analysis of Jurkat using 16495-1-AP

  WB result of Moesin antibody (16495-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Moesin transfected Jurkat cells.

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  WB analysis using 16495-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16495-1-AP (Moesin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 16495-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16495-1-AP (Moesin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16495-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16495-1-AP (Moesin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16495-1-AP (Moesin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16495-1-AP (Moesin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16495-1-AP (Moesin antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 16495-1-AP (Moesin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16495-1-AP (Moesin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16495-1-AP (Moesin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16495-1-AP (Moesin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse kidney using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16495-1-AP (Moesin antibody) at dilution of 1:200 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IF Staining of HepG2 using 16495-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Moesin antibody (16495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 16495-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.20ug Anti-Human Moesin (16495-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, Raji cells, SGC-7901 cells, C6 cells
• Positive IHC detected in: mouse kidney tissue, human colon, human ovary tissue, human placenta tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

16495-1-AP targets Moesin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Moesin fusion protein Ag9623
• Full Name: moesin
• Calculated Molecular Weight: 577 aa, 68 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC017293
• Gene Symbol: Moesin
• Gene ID (NCBI): 4478
• RRID: AB_2282374
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Moesin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to growth factor, chemokine, and antigen stimulation. This antibody may cross-react with ezrin or radixin with molecular weights around 68-70 kDa.

Publications

Species	Application	Title
human	IHC	Acta Neuropathol Commun; The amyloid plaque proteome in early onset Alzheimer's disease and Down syndrome.; Authors - Eleanor Drummond
human	WB	Int J Mol Sci; Bidirectional Tumor-Promoting Activities of Macrophage Ezrin.; Authors - Krishnendu Khan ; KD Validated
human	WB	Front Cell Dev Biol; Ectopic Endometrial Cell-Derived Exosomal Moesin Induces Eutopic Endometrial Cell Migration, Enhances Angiogenesis and Cytosolic Inflammation in Lesions Contributes to Endometriosis Progression.; Authors - Maidinaimu Abudula
mouse	IF	Am J Pathol; Elevated Expression of Moesin in Muscular Dystrophies.; Authors - Mark Pines
human	IF	Cell Rep Med; Multicellular ecotypes shape progression of lung adenocarcinoma from ground-glass opacity toward advanced stages; Authors - Yulan Deng
human	WB,IP	Drug Resist Updat; Intercellular adhesion molecule-1 suppresses TMZ chemosensitivity in acquired TMZ-resistant gliomas by increasing assembly of ABCB1 on the membrane; Authors - Xin Zhang