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MSH2 Monoclonal antibody

MSH2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1


human and More (1)







Cat no : 60161-1-Ig


COCA1, FCC1, hMSH2, HNPCC, HNPCC1, LCFS2, MSH2, MutS protein homolog 2

Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells, HCT 116 cells, HepG2 cells, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60161-1-Ig targets MSH2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MSH2 fusion protein Ag7835
Full Name mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberBC021566
Gene Symbol MSH2
Gene ID (NCBI) 4436
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.


Product Specific Protocols
WB protocol for MSH2 antibody 60161-1-IgDownload protocol
IHC protocol for MSH2 antibody 60161-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cancer Immunol Res

Remodeling Chondroitin-6-Sulfate-Mediated Immune Exclusion Enhances Anti-PD-1 Response in Colorectal Cancer with Microsatellite Stability.

Authors - Qijing Wu

Sci Rep

Cold atmospheric plasma stabilizes mismatch repair for effective, uniform treatment of diverse colorectal cancer cell types

Authors - Yuanyuan He

Transl Res

Novel MSH2 and TSC2 variants in a Chinese family with Lynch syndrome and their synergistic impact in urothelial carcinoma

Authors - Mingyang Li