CD20 Polyclonal antibody

CD20 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 24828-1-AP

Synonyms

Leukocyte surface antigen Leu-16, Bp35, B-lymphocyte surface antigen B1, B-lymphocyte antigen CD20, B1

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Validation Data Gallery

• 

  WB analysis using 24828-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24828-1-AP (CD20 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 24828-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 24828-1-AP (MS4A1,CD20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 24828-1-AP

  IP result of anti-CD20 (IP:24828-1-AP, 4ug; Detection:24828-1-AP 1:600) with Raji cells lysate 1440ug.

• 

  IHC staining of human tonsillitis using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma slide using 24828-1-AP (MS4A1,CD20 Antibody) at dilution of 1:50.

• 

  IHC staining of human lymphoma using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma slide using 24828-1-AP (MS4A1,CD20 Antibody) at dilution of 1:50.

• 

  IHC staining of human tonsillitis using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24828-1-AP (MS4A1,CD20 Antibody) at dilution of 1:50.

• 

  IHC staining of human tonsillitis using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24828-1-AP (MS4A1,CD20 Antibody) at dilution of 1:50.

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (24828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD3 antibody (60181-1-Ig, Clone: 3F3A1, red).

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (24828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD3 antibody (60181-1-Ig, Clone: 3F3A1, red).

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (24828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD3 antibody (60181-1-Ig, Clone: 3F3A1, red).

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 24828-1-AP (CD20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 24828-1-AP (CD20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 24828-1-AP (CD20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PD-1/CD279 mouse mAb (66220-1-Ig) at dilution of 1:50 and CD20 rabbit pAb (24828-1-AP) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66220-1-Ig, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) for 24828-1-AP.

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PD-1/CD279 mouse mAb (66220-1-Ig) at dilution of 1:50 and CD20 rabbit pAb (24828-1-AP) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66220-1-Ig, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) for 24828-1-AP.

• 

  IF Staining of Raji using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD20 antibody (24828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Daudi cells, Raji cells, Ramos cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human tonsillitis tissue, human breast cancer tissue, human colon cancer tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue, Raji cells
• Positive IF/ICC detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 4 publications below

Product Information

24828-1-AP targets CD20 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD20 fusion protein Ag17309
• Full Name: membrane-spanning 4-domains, subfamily A, member 1
• Calculated Molecular Weight: 297 aa, 33 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC002807
• Gene Symbol: CD20
• Gene ID (NCBI): 931
• RRID: AB_2879747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.

Publications

Species	Application	Title
human	IHC	J Immunother Cancer; Infiltrating pattern and prognostic value of tertiary lymphoid structures in resected non-functional pancreatic neuroendocrine tumors.; Authors - Wu-Hu Zhang
human	IHC	Clin Exp Immunol; CMTM6: increased circulating level and up-regulated expression in labial salivary glands in patients with primary Sjogren's syndrome.; Authors - Sirui Qian
human	WB	Acta Physiol (Oxf); Acute physical exercise and long-term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells.; Authors - A Zietzer
human	IHC	Heliyon; Diagnostic, prognostic, and immunological roles of CDSN in ovarian cancer; Authors - Huan Zeng
human	IHC	Int J Mol Sci; CXCR4 Expressed by Tumor-Infiltrating B Cells in Gastric Cancer Related to Survival in the Tumor Microenvironment: An Analysis Combining Single-Cell RNA Sequencing with Bulk RNA Sequencing; Authors - Chen Su

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue. Antigen retrieval Tris-EDTA pH 9. Also works by IHC with sodium citrate antigen retrieval.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate

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