MRPS31 Polyclonal antibody

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• KD/KO Validated

MRPS31 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16288-1-AP

Synonyms

Small ribosomal subunit protein mS31, S31mt, MRP-S31, MRP S31, IMOGN38

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Validation Data Gallery

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  WB analysis using 16288-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16288-1-AP (MRPS31 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16288-1-AP

  WB result of MRPS31 antibody (16288-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS31 transfected HeLa cells.

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  WB analysis using 16288-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16288-1-AP (MRPS31 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 16288-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16288-1-AP (MRPS31 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16288-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPS31 antibody (16288-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, HepG2 cells, MCF-7 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16288-1-AP targets MRPS31 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPS31 fusion protein Ag9434
• Full Name: mitochondrial ribosomal protein S31
• Calculated Molecular Weight: 395 aa, 45 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC022045
• Gene Symbol: MRPS31
• Gene ID (NCBI): 10240
• RRID: AB_2297732
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Hum Mol Genet; Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement.; Authors - Juliette Pulman