MRPS27 Polyclonal antibody

• Featured Product
• KD/KO Validated

MRPS27 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

28

Reviews (1)

Cat no : 17280-1-AP

Synonyms

KIAA0264, MRP S27, MRPS27, S27mt

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Validation Data Gallery

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  WB analysis of A431 using 17280-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17280-1-AP (MRPS27 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 17280-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17280-1-AP (MRPS27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 17280-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17280-1-AP (MRPS27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 17280-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17280-1-AP (MRPS27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 17280-1-AP

  IP result of anti-MRPS27 (IP:17280-1-AP, 3ug; Detection:17280-1-AP 1:1000) with A431 cells lysate 1520ug.

• 

  IHC staining of human kidney using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 17280-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MRPS27 rabbit poly (17280-1-AP, Green) and KRT18 mouse mAb (66187-1-Ig, Red) at dilution of 1:50, further stained with Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L) for 17280-1-AP, and Alexa Fluor 594-conjugated Goat Anti-Mouse IgG (H+L) for 66187-1-Ig.

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Tested Applications

• Positive WB detected in: A431 cells, human heart tissue, human skeletal muscle tissue
• Positive IP detected in: A431 cells
• Positive IHC detected in: human kidney tissue, human lung tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 25 publications below
• IF: See 3 publications below

Product Information

17280-1-AP targets MRPS27 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPS27 fusion protein Ag11047
• Full Name: mitochondrial ribosomal protein S27
• Calculated Molecular Weight: 168 aa, 19 kDa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC030521
• Gene Symbol: MRPS27
• Gene ID (NCBI): 23107
• RRID: AB_2180510
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123].

Publications

Species	Application	Title
human	WB	Cell Metab; The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression.; Authors - Antonicka Hana H
human	WB	Nat Commun; RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.; Authors - Uwe Richter
mouse	WB	J Clin Invest; AFG3L2 supports mitochondrial protein synthesis and Purkinje cell survival.; Authors - Almajan Eva R ER
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia
mouse	WB	J Cell Biol; Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.; Authors - Uwe Richter
human	WB	Proc Natl Acad Sci U S A; Mitochondrial ribosomal protein L12 selectively associates with human mitochondrial RNA polymerase to activate transcription.; Authors - Surovtseva Yulia V YV

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xhuljana (Verified Customer) (03-01-2024)

WB in ripa lysate C2C12 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12 cells

MRPS27 Antibody Western Blot validation (1:1000 dilution) in C2C12 cells (Cat no:17280-1-AP)