MMP3 Recombinant antibody, PBS Only (Detector)

MMP3 Recombinant Antibody for Cytometric bead array, Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

230535G8

Cat no : 82984-4-PBS

Synonyms

SL-1, MMP-3, MMP 3, Matrix metalloproteinase-3, EC:3.4.24.17

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Validation Data Gallery

• 

  WB analysis using 82984-4-RR (same clone as 82984-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82984-4-RR (MMP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82984-4-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 82984-4-RR (same clone as 82984-4-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug MMP3 Recombinant antibody (82984-4-RR, Clone:230535G8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82984-4-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00083-3

  Cytometric bead array standard curve ofMP00083-3, MMP3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82984-1-PBS. Detection antibody: 82984-4-PBS. Standard: Eg0382. Range: 0.625-20 ng/mL.

• 

  Affinity and Kinetic Characterization of 82984-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82984-4-RR against Human MMP3 were performed. The affinity constant is 0.348 nM.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82984-4-PBS targets MMP3 as part of a matched antibody pair:

MP00083-3: 82984-1-PBS capture and 82984-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: matrix metallopeptidase 3 (stromelysin 1, progelatinase)
• GenBank Accession Number: BC074815
• Gene Symbol: MMP3
• Gene ID (NCBI): 4314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: 100% PBS pH 7.3
• Storage Conditions: Store at -80°C.