MMP14 / MT1-MMP Polyclonal antibody, PBS Only

MMP14 / MT1-MMP Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14552-1-PBS

Synonyms

MMP14, Membrane-type-1 matrix metalloproteinase, Membrane-type matrix metalloproteinase 1, Matrix metalloproteinase-14, Matrix metalloproteinase 14

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Validation Data Gallery

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  WB analysis of HepG2 using 14552-1-AP (same clone as 14552-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14552-1-AP (MT1-MMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14552-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 14552-1-AP (same clone as 14552-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 14552-1-AP (MT1-MMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14552-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 14552-1-AP (same clone as 14552-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14552-1-AP (MT1-MMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14552-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human colon using 14552-1-AP (same clone as 14552-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 14552-1-AP (MT1-MMP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14552-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14552-1-AP (same clone as 14552-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14552-1-AP (MT1-MMP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14552-1-PBS in a different storage buffer formulation.

• 

  IF Staining of COLO 320 using 14552-1-AP (same clone as 14552-1-PBS)

  Immunofluorescent analysis of COLO 320 cells using 14552-1-AP (MT1-MMP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14552-1-PBS in a different storage buffer formulation.

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Product Information

14552-1-PBS targets MMP14 / MT1-MMP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MMP14 / MT1-MMP fusion protein Ag5965
• Full Name: matrix metallopeptidase 14 (membrane-inserted)
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66 kDa, 45-50 kDa
• GenBank Accession Number: BC064803
• Gene Symbol: MMP14
• Gene ID (NCBI): 4323
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MMP14, also named as MT1-MMP, is a key matrix metalloproteinase (MMP) family member which plays a crucial role in tumor growth, invasion and metastasis. MT1-MMP is a cell membrane-bound proteinase, and it enhances degradation of collagen IV, a major component of the basement membrane, by forming a complex with tissue inhibitor of metalloproteinase-2 (TIMP-2) to activate pro-MMP-2. MT1-MMP can influence venous invasion, intrahepatic metastasis , and patient outcome in hepatocellular carcinoma (HCC). MT1-MMP was reported to be present in centromere and could lead to chromosome instability in MDCK cells, indicating that MT1-MMP may have more novel functions in the intracellular compartments. In western blotting, pro-MMP14 (65 kDa) and MMP14 (51 kDa) bands showed with the truncated MMP14 (45, 42, 35, 20 kDa) forms (PMID:12097451).