MKRN3 Polyclonal antibody

MKRN3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18349-1-AP

Synonyms

D15S9, makorin ring finger protein 3, MKRN3, RING finger protein 63, RNF63, ZFP127, Zinc finger protein 127, ZNF127

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Validation Data Gallery

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  WB analysis using 18349-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18349-1-AP (MKRN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 18349-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18349-1-AP (MKRN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 18349-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18349-1-AP (MKRN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 18349-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using MKRN3 antibody (18349-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18349-1-AP targets MKRN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MKRN3 fusion protein Ag13167
• Full Name: makorin ring finger protein 3
• Calculated Molecular Weight: 507 aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC044639
• Gene Symbol: MKRN3
• Gene ID (NCBI): 7681
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.