MITD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MITD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 17264-1-AP

Synonyms

MIT domain-containing protein 1

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Validation Data Gallery

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  WB analysis using 17264-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17264-1-AP (MITD1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17264-1-AP

  IP result of anti-MITD1 (IP:17264-1-AP, 4ug; Detection:17264-1-AP 1:500) with HeLa cells lysate 1080ug.

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  IHC staining of human kidney using 17264-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17264-1-AP (MITD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17264-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17264-1-AP (MITD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17264-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17264-1-AP (MITD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 17264-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MITD1 antibody (17264-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, MOLT-4 cells, mouse kidney tissue, rat kidney tissue, rat brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human kidney tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

17264-1-AP targets MITD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MITD1 fusion protein Ag10998
• Full Name: MIT, microtubule interacting and transport, domain containing 1
• Calculated Molecular Weight: 249 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC018453
• Gene Symbol: MITD1
• Gene ID (NCBI): 129531
• RRID: AB_2297522
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; ESCRT-III binding protein MITD1 is involved in cytokinesis and has an unanticipated PLD fold that binds membranes.; Authors - Hadders Michael A MA
human	WB,IHC,IP	Oncogene; The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axis; Authors - Yapeng Wang ; KD Validated
human	WB,IF	Mol Biol Cell; MITD1 is recruited to midbodies by ESCRT-III and participates in cytokinesis.; Authors - Lee Seongju S ; KD Validated
human	WB,IHC	Cells; Pan-Cancer Analysis of the Prognostic and Immunotherapeutic Value of MITD1; Authors - Shiqiang Dong