MFN2 Recombinant antibody

MFN2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A5

Cat no : 82673-2-RR

Synonyms

MARF, KIAA0214, HSG, EC:3.6.5.-, CPRP1

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Validation Data Gallery

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  WB analysis using 82673-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82673-2-RR (MFN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 82673-2-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82673-2-RR (MFN2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 82673-2-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82673-2-RR (MFN2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 82673-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MFN2 antibody (82673-2-RR, Clone: 2A5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  Affinity and Kinetic Characterization of 82673-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82673-2-RR against Human MFN2 were performed. The affinity constant is 19.7 nM.

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Tested Applications

• Positive WB detected in: HepG2 cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82673-2-RR targets MFN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MFN2 fusion protein Ag2845
• Full Name: mitofusin 2
• Calculated Molecular Weight: 757 aa, 86 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC017061
• Gene Symbol: MFN2
• Gene ID (NCBI): 9927
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitofusin-2 (MFN2), a mitochondrial membrane protein, is a dynamin-like GTPase embedded in the outer mitochondrial membrane (OMM) which plays a central role in regulating mitochondrial fusion and cell metabolism (PMID: 35399520; 34026850). There are two human mitofusin isoforms MFN1 and MFN2, MFN1 and MFN2 mediate outer membrane fusion, OPA1 is involved in inner membrane fusion, and DRP1 is responsible for mitochondrial fission (PMID: 17084350; 35399520). It has been shown that MFN2 participates in multiple cellular biological processes under physical and pathological conditions. Apart from mitochondria fusion, MFN2 is also involved in mitochondrial-ER contacts (MERCs), mitophagy, cellular apoptosis, and proliferation (PMID: 34026850).