MFAP5 Polyclonal antibody

• Featured Product
• KD/KO Validated

MFAP5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 15727-1-AP

Synonyms

MAGP 2, MAGP2, MFAP 5, MFAP5, MP25

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Validation Data Gallery

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  WB analysis of HeLa using 15727-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15727-1-AP (MFAP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 15727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15727-1-AP (MFAP5 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 15727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15727-1-AP (MFAP5 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 15727-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15727-1-AP (MFAP5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 15727-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15727-1-AP (MFAP5 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human colon tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 5 publications below

Product Information

15727-1-AP targets MFAP5 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MFAP5 fusion protein Ag8467
• Full Name: microfibrillar associated protein 5
• Calculated Molecular Weight: 173 aa, 20 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC005901
• Gene Symbol: MFAP5
• Gene ID (NCBI): 8076
• RRID: AB_2266295
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB, IHC	Hepatology; YAP promotes proliferation, chemoresistance and angiogenesis in human cholangiocarcinoma via TEAD transcription factors.; Authors - Patricia Marti ; KD Validated
human	IHC	Clin Transl Med; Single-cell transcriptomes reveal heterogeneity of high-grade serous ovarian carcinoma.; Authors - Qian Hao
human	IHC	Front Oncol; DPP6 and MFAP5 are associated with immune infiltration as diagnostic biomarkers in distinguishing uterine leiomyosarcoma from leiomyoma; Authors - Yumin Ke
human	IHC	Hum Cell; Development of a CAFs-related gene signature to predict survival and drug response in bladder cancer.; Authors - Zhao Zhang
human	WB	Clin Transl Oncol; Stromal fibroblast-derived MFAP5 promotes the invasion and migration of breast cancer cells via Notch1/slug signaling.; Authors - Z Chen ; KD Validated
human	IHC	Int J Mol Sci; Microfibril Associated Protein 5 (MFAP5) Is Related to Survival of Ovarian Cancer Patients but Not Useful as a Prognostic Biomarker; Authors - Katarzyna Aleksandra Kujawa