MEN1 Monoclonal antibody, PBS Only (Capture/Detector)

MEN1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A6A11

Cat no : 68473-1-PBS

Synonyms

SCG2, Menin 1, Menin, MEAI, 3A6A11

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Validation Data Gallery

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  WB analysis using 68473-1-Ig (same clone as 68473-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68473-1-Ig (MEN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68473-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68473-1-Ig (same clone as 68473-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68473-1-Ig (MEN1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68473-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68473-1-Ig (same clone as 68473-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68473-1-Ig (MEN1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68473-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50886-1

  Cytometric bead array standard curve of MP50886-1, MEN1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68473-2-PBS. Detection antibody: 68473-1-PBS. Standard:Ag7566. Range: 1.563-200 ng/mL.

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  Cytometric bead array standard curve of MP50886-2

  Cytometric bead array standard curve of MP50886-2, MEN1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68473-1-PBS. Detection antibody: 68473-3-PBS. Standard:null. Range: null.

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Product Information

68473-1-PBS targets MEN1 as part of a matched antibody pair:

MP50886-1: 68473-2-PBS capture and 68473-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MEN1 fusion protein Ag7566
• Full Name: multiple endocrine neoplasia I
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC002544
• Gene Symbol: MEN1
• Gene ID (NCBI): 4221
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MEN1, also named as Menin or MEAI, is a tumor suppressor protein encoded by the MEN1 gene. It is located in nucleus and ubiquitous expression in spleen and lymph node. The calculated molecular weight of MEN1 is 68 kDa. Its inactivation has been implicated in tumors of many endocrine tissues. Germline inactivating mutations of MEN1 gene are the main cause of multiple endocrine neoplasia type 1 (MEN1), a syndrome characterized by tumors in the pituitary gland, the parathyroid glands, and the enteropancreatic neuroendocrine tissues. Recent study shows astroglia menin deficiency leads to depressive-like behaviors in mice.