MEK1-Specific Polyclonal antibody

MEK1-Specific Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 51080-1-AP

Synonyms

ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK 1, MEK1, MEK1-Specific, MKK1, PRKMK1

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Validation Data Gallery

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  IHC staining of human endometrial cancer using 51080-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 51080-1-AP (MEK1-Specific antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human endometrial cancer using 51080-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 51080-1-AP (MEK1-Specific antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 51080-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 51080-1-AP (MEK1-Specific antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 51080-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 51080-1-AP (MEK1-Specific antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human endometrial cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below

Product Information

51080-1-AP targets MEK1-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, Eriocheir sinensis
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase kinase 1
• Calculated Molecular Weight: 43 kDa
• GenBank Accession Number: BC139729
• Gene Symbol: MEK1
• Gene ID (NCBI): 5604
• RRID: AB_2881242
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEK1 is also named as MAP2K1 (mitogen-activated protein kinase kinase 1), MAPKK1, PRKMK1, MKK1 and belongs to the MAP kinase kinase subfamily. It is a dual-specificity kinase that mediate ERK1 and ERK2 activation during adhesion and growth factor signaling(PMID:19219045). It also plays an essential role in extra-embryonic ectoderm during placentogenesis. MEK1 has 2 isoforms produced by alternative splicing with the molecular weight of 43 kDa and 41 kDa. It can be phosphorylated(PMID:21144847). This antibody is specific to MEK1.

Publications

Species	Application	Title
mouse		J Ethnopharmacol; Classical prescription Floris Sophorae Powder treat colorectal cancer by regulating KRAS/MEK-ERK signaling pathway; Authors - Li-Ying Han
human	WB	Cancer Cell Int; MiR-155-5p suppresses SOX1 to promote proliferation of cholangiocarcinoma via RAF/MEK/ERK pathway.; Authors - Da Wang
human	WB	Cancer Cell Int; 4,5-Dimethoxycanthin-6-one is a novel LSD1 inhibitor that inhibits proliferation of glioblastoma cells and induces apoptosis and pyroptosis.; Authors - Wei Li
human	WB	Mol Cell Probes; Elevated PGT promotes proliferation and inhibits cell apoptosis in preeclampsia by Erk signaling pathway; Authors - Huiyuan Pang
human	WB	PLoS One; Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.; Authors - Xu Jiang
human	WB	Onco Targets Ther; FANCI Cooperates with IMPDH2 to Promote Lung Adenocarcinoma Tumor Growth via a MEK/ERK/MMPs Pathway.; Authors - Pengchao Zheng