MDH1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MDH1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

25

Cat no : 15904-1-AP

Synonyms

MDHA, KAR, EC:1.1.1.96, EC:1.1.1.37, Aromatic alpha-keto acid reductase

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Validation Data Gallery

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  WB analysis of HepG2 using 15904-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15904-1-AP (MDH1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15904-1-AP

  WB result of MDH1 antibody (15904-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MDH1 transfected HEK-293 cells.

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  WB analysis of HL-60 using 15904-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15904-1-AP (MDH1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15904-1-AP

  IP result of anti-MDH1 (IP:15904-1-AP, 4ug; Detection:15904-1-AP 1:10000) with HepG2 cells lysate 960 ug.

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  IHC staining of human renal cell carcinoma using 15904-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15904-1-AP (MDH1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 15904-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15904-1-AP (MDH1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15904-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15904-1-AP (MDH1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15904-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15904-1-AP (MDH1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 15904-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 15904-1-AP (MDH1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 15904-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 15904-1-AP (MDH1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15904-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15904-1-AP (MDH1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15904-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15904-1-AP (MDH1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15904-1-AP

  Immunofluorescent analysis of HepG2 cells, using MDH1 antibody 15904-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 15904-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human MDH1 (15904-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HepG2 cells, HL-60 cells, HEK-293 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human renal cell carcinoma tissue, human liver cancer tissue, rat heart tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 22 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 4 publications below

Product Information

15904-1-AP targets MDH1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MDH1 fusion protein Ag8744
• Full Name: malate dehydrogenase 1, NAD (soluble)
• Calculated Molecular Weight: 334 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC001484
• Gene Symbol: MDH1
• Gene ID (NCBI): 4190
• RRID: AB_2143279
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme.(PMID:10693743)

Publications

Species	Application	Title
human	WB	Cell; An Essential Role of the Mitochondrial Electron Transport Chain in Cell Proliferation Is to Enable Aspartate Synthesis.; Authors - Kıvanç Birsoy ; KO Validated
human	WB	Cell Res; The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.; Authors - Jia-Yuan Zhang
mouse	WB	Blood; MDH1-mediated malate-aspartate NADH shuttle maintains the activity levels of fetal liver hematopoietic stem cells.; Authors - Hao Gu
human	WB	J Exp Med; Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.; Authors - Miao Liu
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
human	WB	Nat Commun; Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.; Authors - Xiaoyu Ma

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C