|Positive WB detected in||HEK-293 cells, PC-3 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Sample-dependent, check data in validation data gallery|
10426-1-AP targets MAX in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MAX fusion protein Ag0680|
|Full Name||MYC associated factor X|
|Calculated molecular weight||18 kDa|
|Observed molecular weight||22 kDa|
|GenBank accession number||BC003525|
|Gene ID (NCBI)||4149|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.
MAX Functions as a Tumor Suppressor and Rewires Metabolism in Small Cell Lung Cancer.
A MYC inhibitor selectively alters the MYC and MAX cistromes and modulates the epigenomic landscape to regulate target gene expression.
PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.
Loss of MGA repression mediated by an atypical polycomb complex promotes tumor progression and invasiveness.
Cell Commun Signal
Novel dual-targeting c-Myc inhibitor D347-2761 represses myeloma growth via blocking c-Myc/Max heterodimerization and disturbing its stability.
J Biol Chem
Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1.