MAX Polyclonal antibody

• Featured Product
• KD/KO Validated

MAX Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 10426-1-AP

Synonyms

bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8, MAX, MYC associated factor X, orf1, Protein max

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Validation Data Gallery

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  WB analysis of HEK-293 using 10426-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10426-1-AP (MAX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10426-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10426-1-AP (MAX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10426-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10426-1-AP (MAX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• ChIP: See 4 publications below

Product Information

10426-1-AP targets MAX in WB, IHC, IF, chIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAX fusion protein Ag0680
• Full Name: MYC associated factor X
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC003525
• Gene Symbol: MAX
• Gene ID (NCBI): 4149
• RRID: AB_2141660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.

Publications

Species	Application	Title
mouse	WB	Cancer Cell; MAX Functions as a Tumor Suppressor and Rewires Metabolism in Small Cell Lung Cancer.; Authors - Arnaud Augert ; KO Validated
human	WB	Sci Adv; A MYC inhibitor selectively alters the MYC and MAX cistromes and modulates the epigenomic landscape to regulate target gene expression.; Authors - Austin G Holmes
human	WB	Nat Commun; PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.; Authors - Xianchun Lan
human	WB,ChIP	Elife; Loss of MGA repression mediated by an atypical polycomb complex promotes tumor progression and invasiveness.; Authors - Haritha Mathsyaraja
human	WB	Cell Commun Signal; Novel dual-targeting c-Myc inhibitor D347-2761 represses myeloma growth via blocking c-Myc/Max heterodimerization and disturbing its stability.; Authors - Ruosi Yao
human	chIP	J Biol Chem; Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1.; Authors - Jianyin Long