MAVS; VISA Monoclonal antibody, PBS Only

MAVS; VISA Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A8E9

Cat no : 66911-1-PBS

Synonyms

CARDIF, DKFZp547C224, DKFZp666M015, FLJ27482, FLJ35386, FLJ38051, FLJ41962, IPS 1, IPS1, KIAA1271, MAVS, MAVS; VISA, VISA

---

Validation Data Gallery

• 

  WB analysis of A431 using 66911-1-Ig (same clone as 66911-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66911-1-Ig (same clone as 66911-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66911-1-Ig (same clone as 66911-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66911-1-Ig (same clone as 66911-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 66911-1-Ig (same clone as 66911-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66911-1-Ig (same clone as 66911-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66911-1-Ig (same clone as 66911-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:1100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66911-1-Ig (same clone as 66911-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:1100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66911-1-PBS targets MAVS; VISA in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MAVS; VISA fusion protein Ag5949
• Full Name: mitochondrial antiviral signaling protein
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 50-55 kDa, 70-75 kDa
• GenBank Accession Number: BC044952
• Gene Symbol: MAVS
• Gene ID (NCBI): 57506
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN beta promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.