Matrilin 2 Polyclonal antibody

Matrilin 2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 24064-1-AP

Synonyms

MATN2, Matrilin 2

---

Validation Data Gallery

• 

  WB analysis of NIH/3T3 using 24064-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24064-1-AP (Matrilin 2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 24064-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24064-1-AP (Matrilin 2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24064-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24064-1-AP (Matrilin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: NIH/3T3 cells, HEK-293 cells, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 24064-1-AP is Matrilin 2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Matrilin 2 fusion protein Ag21113
• Full Name: matrilin 2
• Calculated Molecular Weight: 107 kDa
• Observed Molecular Weight: 120-150 kDa
• GenBank Accession Number: BC010444
• Gene Symbol: Matrilin 2
• Gene ID (NCBI): 4147
• RRID: AB_2879422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The matrilins represent a new family of oligomeric non-collagenous extracellular matrix proteins that are assumed to act as adapter molecules connecting other proteins and proteoglycans in the extracellular matrix (PMID: 17513098). Four matrilins (matrilin-1, -2, -3, and -4) have been identified. They all share a structure made up of von Willebrand factor A domains, EGF-like domains and a coiled-coil alpha-helical module (PMID: 18386166). Matrilin-1 and matrilin-3 are expressed mainly in cartilage, while matrilin-2 and matrilin-4 show broader tissue distribution. Matrilin-2 is the largest member of the family. It incorporates into loose and dense connective tissue and becomes associated with some basement membranes (PMID: 17513098).

Publications

Species	Application	Title
zebrafish	WB	Sci Adv; A basement membrane discovery pipeline uncovers network complexity, regulators, and human disease associations.; Authors - Ranjay Jayadev ; KD Validated
mouse	WB,IF	J Mol Med (Berl); Valproic acid modulates collagen architecture in the postoperative conjunctival scar.; Authors - Li-Fong Seet
human	WB	Int J Mol Sci; A Regulator Role for the ATP-Binding Cassette Subfamily C Member 6 Transporter in HepG2 Cells: Effect on the Dynamics of Cell-Cell and Cell-Matrix Interactions; Authors - Ilenia Matera
human	WB,CoIP	Funct Integr Genomics; MATN2 overexpression suppresses tumor growth in ovarian cancer via PTEN/PI3K/AKT pathway; Authors - Jingbo Liu