MARCH5 Polyclonal antibody

• 

  WB analysis of HeLa using 12213-1-AP

  WB result of MARCH5 antibody (12213-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-MARCH5 transfected HeLa cells.

• 

  WB analysis using 12213-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12213-1-AP (MARCH5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 12213-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12213-1-AP (MARCH5 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 12213-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12213-1-AP (MARCH5 Antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

MARCH5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12213-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

MARCH V, MARCH5, Mitochondrial ubiquitin ligase, MITOL, RING finger protein 153, RNF153

---

Tested Applications

• Positive WB detected in: HeLa cells, U-937 cells
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12213-1-AP targets MARCH5 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MARCH5 fusion protein Ag2806
• Full Name: membrane-associated ring finger (C3HC4) 5
• Calculated Molecular Weight: 278 aa, 31 kDa
• Observed Molecular Weight: 31 kDa, 65-70 kDa
• GenBank Accession Number: BC015480
• Gene Symbol: MARCH5
• Gene ID (NCBI): 54708
• RRID: AB_10638602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NX47
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MARCH5(E3 ubiquitin-protein ligase MARCH5) is a 31 kDa protein also named as RNF153. This protein plays a protective role against mitochondrial dysfunction caused by the mitochondrial accumulation of mSOD1 via the ubiquitin-proteasome pathway (PMID:19741096). And it may regulate MAP1B LC1 function in mitochondria by controlling mitochondrial aggregation via mitochondrial LC1 levels(PMID:22308378). MEARCH5 can exsit as a homodimer with MW 65-70 kDa.

Publications

Species	Application	Title
human	WB	Clin Transl Med; FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression.; Authors - Lei Miao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Tom (Verified Customer) (04-09-2021)

HEK293 whole cell lysates ran on denaturing acrylamide gel and trasnferred to nitrocellulose membrane. Incubated overnight with antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293

MARCH5 Antibody Western Blot validation (1/1000 dilution) in HEK293 (Cat no:12213-1-AP)