MAP3K6 Polyclonal antibody

MAP3K6 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16212-1-AP

Synonyms

Mitogen-activated protein kinase kinase kinase 6, MEKK6, MAPKKK6, EC:2.7.11.25, ASK2

---

Validation Data Gallery

• 

  IHC staining of human liver using 16212-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16212-1-AP (MAP3K6 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 16212-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16212-1-AP (MAP3K6 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 16212-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16212-1-AP (MAP3K6 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 16212-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16212-1-AP (MAP3K6 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 16212-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16212-1-AP (MAP3K6 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 16212-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16212-1-AP (MAP3K6 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 16212-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16212-1-AP (MAP3K6 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 16212-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16212-1-AP (MAP3K6 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 16212-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MAP3K6 antibody (16212-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive IHC detected in: human liver tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16212-1-AP targets MAP3K6 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAP3K6 fusion protein Ag9206
• Full Name: mitogen-activated protein kinase kinase kinase 6
• Calculated Molecular Weight: 1148aa,128 kDa; 1011aa,113 kDa; 797aa,89 kDa
• Observed Molecular Weight: 88 kDa
• GenBank Accession Number: BC015914
• Gene Symbol: MAP3K6
• Gene ID (NCBI): 9064
• RRID: AB_2878229
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.