LC3C-Specific Polyclonal antibody

LC3C-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 18726-1-AP

Synonyms

LC3C, MAP1A/MAP1B LC3 C, MAP1A/MAP1B light chain 3 C, MAP1LC3C

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Validation Data Gallery

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  WB analysis of Recombinant protein using 18726-1-AP

  WB results of LC3C-specific antibody with Recombinant protein LC3A, LC3B and LC3C, by Dr. Christos Zois.

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  IHC staining of human liver using 18726-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18726-1-AP (LC3C-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 18726-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18726-1-AP (LC3C-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Recombinant protein
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

18726-1-AP targets LC3C-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: microtubule-associated protein 1 light chain 3 gamma
• Calculated Molecular Weight: 17 kDa
• GenBank Accession Number: NM_001004343
• Gene Symbol: LC3C
• Gene ID (NCBI): 440738
• RRID: AB_2878557
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LC3C, also named as MAP1LC3C, belongs to the MAP1 LC3 family. It is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3C is produced by the proteolytic cleavage after the conserved C-terminal Gly residue. The antibody is specific to the prepeptide of LC3C.

Publications

Species	Application	Title
human	WB,IF	Autophagy; Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy.; Authors - Jonathan A Stefely
mouse	IF	J Immunol; Cutting Edge: Phagosome-associated Autophagosomes Containing Antigens and Proteasomes Drive TAP-Independent Cross-Presentation; Authors - Debrup Sengupta
human	WB,IP	Front Pharmacol; SC75741, A Novel c-Abl Inhibitor, Promotes the Clearance of TDP25 Aggregates via ATG5-Dependent Autophagy Pathway; Authors - Dongheng Zhou
human	WB, IF	PLoS One; Autophagosome Proteins LC3A, LC3B and LC3C Have Distinct Subcellular Distribution Kinetics and Expression in Cancer Cell Lines.; Authors - Michael I Koukourakis
human	WB	Adv Exp Med Biol; Microtubule-Associated Protein 1 Light Chain 3 (LC3) Isoforms in RPE and Retina.; Authors - Anuradha Dhingra
human	WB	Dev Cell; V-ATPase recruitment to ER exit sites switches COPII-mediated transport to lysosomal degradation; Authors - Yiwei Sun