Lysozyme Polyclonal antibody, PBS Only

Lysozyme Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15013-1-PBS

Synonyms

LYZ, Muramidase, LZM, Lysozyme C, EC:3.2.1.17

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Validation Data Gallery

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  WB analysis of mouse lung using 15013-1-AP (same clone as 15013-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15013-1-AP (LYZ antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15013-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 15013-1-AP (same clone as 15013-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 15013-1-AP (LYZ antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15013-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 15013-1-AP (same clone as 15013-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15013-1-AP (LYZ antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15013-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 15013-1-AP (same clone as 15013-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 15013-1-AP (Lysozyme antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15013-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 15013-1-AP (same clone as 15013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15013-1-AP (Lysozyme antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15013-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 15013-1-AP (same clone as 15013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15013-1-AP (Lysozyme antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15013-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 15013-1-AP (same clone as 15013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15013-1-AP (Lysozyme antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15013-1-PBS in a different storage buffer formulation.

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Product Information

15013-1-PBS targets Lysozyme in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Lysozyme fusion protein Ag6917
• Full Name: lysozyme (renal amyloidosis)
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 15-17 kDa
• GenBank Accession Number: BC004147
• Gene Symbol: Lysozyme
• Gene ID (NCBI): 4069
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.