Tested Applications
| Positive FC detected in | mouse bone marrow cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PE-65078 targets Ly-6G in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Ly-6G transfected EL-4J cell line Predict reactive species |
| Full Name | lymphocyte antigen 6 complex, locus G |
| GenBank Accession Number | X70920 |
| Gene Symbol | Ly-6G |
| Gene ID (NCBI) | 546644 |
| RRID | AB_2883874 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
| Excitation Laser | Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P35461 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The 1A8 mAb is specific for Ly-6G (PMID: 8360469).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE Ly-6G antibody PE-65078 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Mouse Ly-6G (1A8), catalog number PE-65078, has 45 citations appearing in journals including Cell, Nature Communications, Advanced Materials, ACS Nano, Advanced Science, Oncogene, Free Radical Biology and Medicine, Journal of Neuroinflammation, Phytomedicine, and International Immunopharmacology. Research fields span tumor immunity, sepsis-induced lung injury, atherosclerosis, neuroinflammation, ischemic stroke, liver ischemia-reperfusion injury, rheumatoid arthritis, psoriasis, inflammatory bowel disease, and acute pancreatitis.
| Species | Application | Title |
|---|---|---|
Phytomedicine Shen Yuan Yi Qi Huo Xue capsules inhibit neutrophil NETosis by regulating the PAD4/NLRP3 signaling pathway to prevent heart failure after myocardial infarction. | ||
ACS Appl Mater Interfaces Disrupting Neutrophil Extracellular Traps with Targeted Cerium Oxide Nanoparticles Ameliorates Diabetic Periodontitis. | ||
Cell Signal Neonatal necrotizing enterocolitis attenuates adult asthma via eosinophil ferroptosis |
