Lamin B1 Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

Lamin B1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5H10

Cat no : 80906-1-PBS

Synonyms

LMNB1, LMNB, LMN2, Lamin-B1, laminB1

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Validation Data Gallery

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  WB analysis using 80906-1-RR (same clone as 80906-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80906-1-RR (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 80906-1-RR (same clone as 80906-1-PBS)

  WB result of Lamin B1 antibody (80906-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin B1 transfected HeLa cells. This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 80906-1-RR (same clone as 80906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 80906-1-RR (Lamin B1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 80906-1-RR (same clone as 80906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80906-1-RR (Lamin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 80906-1-RR (same clone as 80906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80906-1-RR (Lamin B1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 80906-1-RR (same clone as 80906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80906-1-RR (Lamin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 80906-1-RR (same clone as 80906-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B1 antibody (80906-1-RR, Clone: 5H10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 80906-1-RR (same clone as 80906-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B1 antibody (80906-1-RR, Clone: 5H10 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80906-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80906-1-RR against Human Lamin B1 were performed. The affinity constant is 13.8 nM.

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Product Information

80906-1-PBS targets Lamin B1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Lamin B1 fusion protein Ag3631
• Full Name: lamin B1
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC012295
• Gene Symbol: Lamin B1
• Gene ID (NCBI): 4001
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis (PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.