Lamin A/C Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

Lamin A/C Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5I16

Cat no : 81042-1-PBS

Synonyms

LaminA/C, LMNA, LMN1, Lamin-A/C, lamin A

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Validation Data Gallery

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  WB analysis using 81042-1-RR (same clone as 81042-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81042-1-RR (Lamin A/C antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 81042-1-RR (same clone as 81042-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81042-1-RR (Lamin A/C antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 81042-1-RR (same clone as 81042-1-PBS)

  WB result of Lamin A/C antibody (81042-1-RR; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin A/C transfected HeLa cells. This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 81042-1-RR (same clone as 81042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 81042-1-RR (Lamin A/C antibody) at dilution of 1:12000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 81042-1-RR (same clone as 81042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 81042-1-RR (Lamin A/C antibody) at dilution of 1:12000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 81042-1-RR (same clone as 81042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81042-1-RR (Lamin A/C antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 81042-1-RR (same clone as 81042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81042-1-RR (Lamin A/C antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 81042-1-RR (same clone as 81042-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Lamin A/C antibody (81042-1-RR, Clone: 5I16 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81042-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81042-1-RR against Human Lamin A/C were performed. The affinity constant is 92.9 pM.

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Product Information

81042-1-PBS targets Lamin A/C in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Lamin A/C fusion protein Ag0408
• Full Name: lamin A/C
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC003162
• Gene Symbol: Lamin A/C
• Gene ID (NCBI): 4000
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Lamin A/C is also named as LMNA, or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is as an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.