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  • KD/KO Validated

LTBR Polyclonal antibody

LTBR Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 20331-1-AP

Synonyms

TNF RIII, TNF R III, Lymphotoxin-beta receptor, Lymphotoxin beta receptor, LT BETA R



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20331-1-AP targets LTBR in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LTBR fusion protein Ag14065
Full Name lymphotoxin beta receptor (TNFR superfamily, member 3)
Calculated Molecular Weight 435 aa, 47 kDa
Observed Molecular Weight 47-60 kDa
GenBank Accession NumberBC026262
Gene Symbol LTBR
Gene ID (NCBI) 4055
RRIDAB_10694276
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LTBR antibody 20331-1-APDownload protocol
IHC protocol for LTBR antibody 20331-1-APDownload protocol
FC protocol for LTBR antibody 20331-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Mol Ther

Activation of NF-κB-Inducing Kinase in Islet β Cells Causes β Cell Failure and Diabetes.

Authors - Xinzhi Li
humanWB

J Immunol

SNX17 Affects T Cell Activation by Regulating TCR and Integrin Recycling.

Authors - Douglas G Osborne
humanIHC,IF,WB

Am J Pathol

Lymphotoxins Promote the Progression of Human Lymphatic Malformation by Enhancing Lymphatic Endothelial Cell Proliferation.

Authors - Jie-Gang Yang
  • KD Validated

Mol Med Rep

Lymphotoxin β receptor activation promotes mRNA expression of RelA and pro-inflammatory cytokines TNFα and IL-1β in bladder cancer cells.

Authors - Mo Shen
mouseIF

Theranostics

Islet α-cell Inflammation Induced By NF-κB inducing kinase (NIK) Leads to Hypoglycemia, Pancreatitis, Growth Retardation, and Postnatal Death in Mice.

Authors - Xinzhi Li
humanIHC,IF

J Reprod Immunol

A LIGHT-HVEM/LTβR axis contributes to the fibrosis of intrauterine adhesion

Authors - Ayitila Abudukeyoumu