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LTBR Polyclonal antibody
LTBR Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF, FC (Intra), ELISA
Conjugate
Unconjugated
Cat no : 20331-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, mouse brain tissue |
Positive IHC detected in | human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 3 publications below |
IHC | See 2 publications below |
IF | See 4 publications below |
Product Information
20331-1-AP targets LTBR in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | LTBR fusion protein Ag14065 |
Full Name | lymphotoxin beta receptor (TNFR superfamily, member 3) |
Calculated Molecular Weight | 435 aa, 47 kDa |
Observed Molecular Weight | 47-60 kDa |
GenBank Accession Number | BC026262 |
Gene Symbol | LTBR |
Gene ID (NCBI) | 4055 |
RRID | AB_10694276 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for LTBR antibody 20331-1-AP | Download protocol |
IHC protocol for LTBR antibody 20331-1-AP | Download protocol |
FC protocol for LTBR antibody 20331-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Ther Activation of NF-κB-Inducing Kinase in Islet β Cells Causes β Cell Failure and Diabetes. | ||
Am J Pathol Lymphotoxins Promote the Progression of Human Lymphatic Malformation by Enhancing Lymphatic Endothelial Cell Proliferation.
| ||
Mol Med Rep Lymphotoxin β receptor activation promotes mRNA expression of RelA and pro-inflammatory cytokines TNFα and IL-1β in bladder cancer cells. | ||
Theranostics Islet α-cell Inflammation Induced By NF-κB inducing kinase (NIK) Leads to Hypoglycemia, Pancreatitis, Growth Retardation, and Postnatal Death in Mice. | ||
J Reprod Immunol A LIGHT-HVEM/LTβR axis contributes to the fibrosis of intrauterine adhesion |