LSM14A Polyclonal antibody, PBS Only

LSM14A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18336-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 18336-1-AP (same clone as 18336-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18336-1-AP (LSM14A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18336-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 18336-1-AP (same clone as 18336-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18336-1-AP (LSM14A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18336-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 18336-1-AP (same clone as 18336-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 18336-1-AP (LSM14A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18336-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 18336-1-AP (same clone as 18336-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18336-1-AP (LSM14A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18336-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 18336-1-AP (same clone as 18336-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 18336-1-AP (LSM14A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18336-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 18336-1-AP (same clone as 18336-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 18336-1-AP (LSM14A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18336-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 18336-1-AP (same clone as 18336-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 18336-1-AP (LSM14A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18336-1-PBS in a different storage buffer formulation.

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Product Information

18336-1-PBS targets LSM14A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LSM14A fusion protein Ag12997
• Full Name: LSM14A, SCD6 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 463 aa, 51 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC016842
• Gene Symbol: LSM14A
• Gene ID (NCBI): 26065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LSm14A was originally discovered to be a component of the P-bodies, it is a member of the LSm family of proteins, which have been shown to be involved in RNA processing events. The N terminus of LSm14A is a conserved LSm domain, whereas the C terminus contains two domains called "DFDF box" and "FDF_TFG box", respectively.