LRRC2 Polyclonal antibody

LRRC2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28057-1-AP

Synonyms

LRRC2

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Validation Data Gallery

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  WB analysis using 28057-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28057-1-AP (LRRC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 28057-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28057-1-AP (LRRC2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28057-1-AP targets LRRC2 in WB, IHC, ELISA applications and shows reactivity with mouse, rat, mouse samples.

• Tested Reactivity: mouse, rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LRRC2 fusion protein Ag27830
• Full Name: leucine rich repeat containing 2
• Calculated Molecular Weight: 371 aa, 43 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC029118
• Gene Symbol: LRRC2
• Gene ID (NCBI): 79442
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Leucine Rich Repeat Containing 2 (LRRC2) was found to be localized to the mitochondria in human cells and transcriptionally regulated by the mitochondrial master regulator Pgc-1α. It has been reported that Lrrc2 transcript abundance correlates with that of β-MHC, a canonical marker of cardiac hypertrophy in humans, and experimentally demonstrated an elevation in Lrrc2 transcript in vitro and in vivo rodent models of cardiac hypertrophy as well as in patients with dilated cardiomyopathy. (PMID: 28158196)