LRP2-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

LRP2-Specific Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

14

Reviews (2)

Cat no : 19700-1-AP

Synonyms

DBS, Fx1a, Glycoprotein 330, gp330, LRP 2, LRP2, LRP2-Specific, Megalin

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Validation Data Gallery

• 

  WB analysis using 19700-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19700-1-AP (LRP2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 19700-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19700-1-AP (LRP2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19700-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19700-1-AP (LRP2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 19700-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19700-1-AP (LRP2-Specific antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 19700-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19700-1-AP (LRP2-Specific antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human kidney using 19700-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using LRP2-Specific antibody (19700-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human kidney using 19700-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using LRP2-Specific antibody (19700-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human brain tissue, human placenta tissue, HeLa cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 5 publications below

Product Information

19700-1-AP targets LRP2-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: low density lipoprotein-related protein 2
• Calculated Molecular Weight: 522 kDa
• Observed Molecular Weight: 280 kDa
• GenBank Accession Number: NM_004525
• Gene Symbol: LRP2
• Gene ID (NCBI): 4036
• RRID: AB_10640428
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRP2, also named as gp330 and Megalin, belongs to the LDLR family. It acts together with cubilin to mediate HDL endocytosis. LRP2 may participate in regulation of parathyroid-hormone and para-thyroid-hormone-related protein release. Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS). This antibody is specific to LRP2 and detects endogenous LRP2 with an apparent molecular weight 280 kDa, which is smaller than the predicted molecular weight (522 kDa) and probably represent a proteolytic fragment of the large glycoprotein (PMID: 16638803; 8483924).

Publications

Species	Application	Title
mouse	IF	EMBO Rep; Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.; Authors - Ye Chen
human	WB,IHC	Nephrol Dial Transplant; Reduced proximal tubular expression of protein endocytic receptors in proteinuria is associated with urinary receptor shedding.; Authors - Hiwa Fatah
mouse	WB	Development; Mouse early extra-embryonic lineages activate compensatory endocytosis in response to poor maternal nutrition.; Authors - Congshan Sun
mouse	IHC,IF	Metabolism; Sodium-glucose cotransporter 2 inhibition attenuates protein overload in renal proximal tubule via suppression of megalin O-GlcNacylation in progressive diabetic nephropathy.; Authors - Hitomi Otomo
mouse	WB	Mol Ther Nucleic Acids; Gelofusine Attenuates Tubulointerstitial Injury Induced by cRGD-Conjugated siRNA by Regulating the TLR3 Signaling Pathway.; Authors - Bohong Cen
human	WB	Stem Cell Res; Generation of a homozygous LRP2 knockout human embryonic stem cell line (FDCHDPe010-A-56) by CRISPR/Cas9 system.; Authors - Jie You ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Brittney (Verified Customer) (11-29-2018)

Excellent antibody for IHC. Used acidic antigen retrieval on paraffin embedded mouse kidney sections. Developed with DAB substrate.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse Kidney Tissue

LRP2-Specific Antibody Immunohistochemistry, validation (1:100 dilution) in Mouse Kidney Tissue (Cat no:19700-1-AP)

FH

Brittney (Verified Customer) (11-29-2018)

Excellent antibody for IHC. Used acidic antigen retrieval and developed with DAB substrate.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse Kidney Tissue

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