LONP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

LONP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

90

Reviews (1)

Cat no : 15440-1-AP

Synonyms

PIM1, Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON

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Validation Data Gallery

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  WB analysis of Jurkat cells using 15440-1-AP

  WB result of LONP1 antibody (15440-1-AP, 1:6000) with si-Control and si-LONP1 transfected Jurkat cells.

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  WB analysis using 15440-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15440-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15440-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15440-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15440-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 15440-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis of rat lung using 15440-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  IP experiment of mouse heart using 15440-1-AP

  IP result of anti-LONP1 (IP:15440-1-AP, 3ug; Detection:15440-1-AP 1:1000) with mouse heart tissue lysate 9500ug.

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  IHC staining of human lung cancer using 15440-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15440-1-AP (LONP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15440-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15440-1-AP (LONP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15440-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15440-1-AP (LONP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15440-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15440-1-AP (LONP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15440-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LONP1 antibody (15440-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 15440-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LONP1 antibody (15440-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 15440-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15440-1-AP (LONP1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 15440-1-AP

  Immunofluorescent analysis of HepG2 cells using 15440-1-AP (LONP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human heart tissue, rat heart tissue, rat lung tissue, mouse heart tissue, Jurkat cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 11 publications below
• WB: See 81 publications below
• IHC: See 3 publications below
• IF: See 10 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Product Information

15440-1-AP targets LONP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LONP1 fusion protein Ag7196
• Full Name: lon peptidase 1, mitochondrial
• Calculated Molecular Weight: 106 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC000235
• Gene Symbol: LONP1
• Gene ID (NCBI): 9361
• RRID: AB_2137152
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then progressively degraded(PMID:15870080). LONP1 has some isoforms with the MW of 106 kDa, 100 kDa and 86 kDa.

Publications

Species	Application	Title
human	WB	Nature; A cytosolic surveillance mechanism activates the mitochondrial UPR; Authors - F X Reymond Sutandy ; KD Validated
human	WB	Cell Metab; A High-Density Human Mitochondrial Proximity Interaction Network.; Authors - Hana Antonicka
monkey	WB,IF	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König
human	WB	Mol Cell; Circadian Oscillation of Sulfiredoxin in the Mitochondria.; Authors - In Sup Kil ; KD Validated
mouse	WB	Sci Adv; Fidelity of translation initiation is required for coordinated respiratory complex assembly.; Authors - Danielle L Rudler
mouse	WB,IHC	Sci Adv; Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4.; Authors - Qiqi Guo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

B (Verified Customer) (01-28-2023)

It is okay to detect endogenous LONP1 although there are weak non specific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

LONP1 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:15440-1-AP)