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LONP1 Polyclonal antibody
LONP1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 15440-1-AP
Validation Data Gallery
|Positive WB detected in
|HeLa cells, human heart tissue, rat heart tissue, rat lung tissue, mouse heart tissue, Jurkat cells, mouse brain tissue, rat brain tissue
|Positive IP detected in
|mouse heart tissue
|Positive IHC detected in
|human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:1000-1:6000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
15440-1-AP targets LONP1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, monkey
|Host / Isotype
|Rabbit / IgG
|LONP1 fusion protein Ag7196
|lon peptidase 1, mitochondrial
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then progressively degraded(PMID:15870080). LONP1 has some isoforms with the MW of 106 kDa, 100 kDa and 86 kDa.
|Product Specific Protocols
|WB protocol for LONP1 antibody 15440-1-AP
|IHC protocol for LONP1 antibody 15440-1-AP
|IF protocol for LONP1 antibody 15440-1-AP
|IP protocol for LONP1 antibody 15440-1-AP
|Click here to view our Standard Protocols
A cytosolic surveillance mechanism activates the mitochondrial UPR
A High-Density Human Mitochondrial Proximity Interaction Network.
Nat Cell Biol
MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.
Circadian Oscillation of Sulfiredoxin in the Mitochondria.
Fidelity of translation initiation is required for coordinated respiratory complex assembly.
Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
B (Verified Customer) (01-28-2023)
It is okay to detect endogenous LONP1 although there are weak non specific bands.