Tested Applications
| Positive WB detected in | HeLa cells, human heart tissue, rat heart tissue, rat lung tissue, mouse heart tissue, Jurkat cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15440-1-AP targets LONP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7196 Product name: Recombinant human LONP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 614-959 aa of BC000235 Sequence: EQNANFLDHYLDVPVDLSKVLFICTANVTDTIPEPLRDRMEMINVSGYVAQEKLAIAERYLVPQARALCGLDESKAKLSSDVLTLLIKQYCRESGVRNLQKQVEKVLRKSAYKIVSGEAESVEVTPENLQDFVGKPVFTVERMYDVTPPGVVMGLAWTAMGGSTLFVETSLRRPQDKDAKGDKDGSLEVTGQLGEVMKESARIAYTFARAFLMQHAPANDYLVTSHIHLHVPEGATPKDGPSAGCTIVTALLSLAMGRPVRQNLAMTGEVSLTGKILPVGGIKEKTIAAKRAGVTCIVLPAENKKDFYDLAAFITEGLEVHFVEHYREIFDIAFPDEQAEALAVER Predict reactive species |
| Full Name | lon peptidase 1, mitochondrial |
| Calculated Molecular Weight | 106 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC000235 |
| Gene Symbol | LONP1 |
| Gene ID (NCBI) | 9361 |
| RRID | AB_2137152 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P36776 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then progressively degraded(PMID:15870080). LONP1 has some isoforms with the MW of 106 kDa, 100 kDa and 86 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LONP1 antibody 15440-1-AP | Download protocol |
| IHC protocol for LONP1 antibody 15440-1-AP | Download protocol |
| IP protocol for LONP1 antibody 15440-1-AP | Download protocol |
| WB protocol for LONP1 antibody 15440-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-LONP1 antibody (Cat# 15440-1-AP) has accumulated 136 citations across high-impact journals including Nature, Science, Cell Metabolism, Nature Communications, Neuron, EMBO Journal, PNAS, and Molecular Cell. Research spans mitochondrial proteostasis, UPRmt signaling, mitophagy, neurodegeneration (Parkinson's, Alzheimer's), cancer biology, metabolic diseases (diabetes, obesity), cardiovascular pathology, liver fibrosis, ferroptosis, and aging-related disorders.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Tumor-derived DNA drives cancer-associated anemia by promoting reticulocyte clearance. | ||
Science Artemisinins ameliorate polycystic ovarian syndrome by mediating LONP1-CYP11A1 interaction
| ||
Nat Metab LONP1 regulation of mitochondrial protein folding provides insight into beta cell failure in type 2 diabetes
| ||
Cell Stem Cell Context-dependent roles of mitochondrial LONP1 in orchestrating the balance between airway progenitor versus progeny cells
|
Reviews
What customers say
Both reviewers used this antibody for Western Blot. One user (5/5) reported excellent performance for CRISPR knockout validation in HeLa cells at 1:1000 dilution. Another (4/5) found it acceptable for detecting endogenous LONP1 in mouse cortex lysate at 1:2000, though noted weak non-specific bands. Overall, the antibody performs well for its intended target with minor background issues in some applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Victor (Verified Customer) (08-10-2026) | Worked excellent for CRISPR KO validation in HeLa.
 |
B (Verified Customer) (01-28-2023) | It is okay to detect endogenous LONP1 although there are weak non specific bands.
 |
