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  • KD/KO Validated

LONP1 Polyclonal antibody

LONP1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 15440-1-AP


hLON, LON, lon peptidase 1, mitochondrial, Lon protease like protein, LonHS, LONP, LONP1, PIM1, PRSS15, Serine protease 15

Tested Applications

Positive WB detected inHeLa cells, human heart tissue, mouse heart tissue, rat heart tissue, rat lung tissue, Jurkat cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15440-1-AP targets LONP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LONP1 fusion protein Ag7196
Full Name lon peptidase 1, mitochondrial
Calculated molecular weight 106 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC000235
Gene symbol LONP1
Gene ID (NCBI) 9361
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then progressively degraded(PMID:15870080). LONP1 has some isoforms with the MW of 106 kDa, 100 kDa and 86 kDa.


Product Specific Protocols
WB protocol for LONP1 antibody 15440-1-APDownload protocol
IHC protocol for LONP1 antibody 15440-1-APDownload protocol
IF protocol for LONP1 antibody 15440-1-APDownload protocol
IP protocol for LONP1 antibody 15440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biochim Biophys Acta Mol Basis Dis

Mutant huntingtin inhibits the mitochondrial unfolded protein response by impairing ABCB10 mRNA stability.

Authors - Zixing Fu

Acta Neuropathol

Alpha-synuclein suppresses mitochondrial protease ClpP to trigger mitochondrial oxidative damage and neurotoxicity.

Authors - Di Hu

PLoS Genet

TDP-43 induces mitochondrial damage and activates the mitochondrial unfolded protein response.

Authors - Peng Wang


CDDO-Me Selectively Attenuates CA1 Neuronal Death Induced by Status Epilepticus via Facilitating Mitochondrial Fission Independent of LONP1.

Authors - Ji-Eun Kim
  • KD Validated


TRPC6-Mediated ERK1/2 Activation Increases Dentate Granule Cell Resistance to Status Epilepticus Via Regulating Lon Protease-1 Expression and Mitochondrial Dynamics.

Authors - Ji-Eun Kim

Sci Adv

Fidelity of translation initiation is required for coordinated respiratory complex assembly.

Authors - Danielle L Rudler