AGPHD1 Polyclonal antibody

AGPHD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26070-1-AP

Synonyms

5-hydroxy-L-lysine kinase, AGPHD1, Hydroxylysine kinase, HYKK

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Validation Data Gallery

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  WB analysis of mouse liver using 26070-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26070-1-AP (LOC123688 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26070-1-AP is AGPHD1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AGPHD1 fusion protein Ag23420
• Full Name: similar to RIKEN cDNA C630028N24 gene
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC132753
• Gene Symbol: AGPHD1/HYKK
• Gene ID (NCBI): 123688
• RRID: AB_2880361
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AGPHD1 encodes the 5-hydroxy-L-lysine kinase which catalyzes the GTP-dependent phosphorylation of 5-hydroxy-L-lysine. It is primarily present in liver and kidneys, suggesting its major role in degrading 5Hyl derived from food. Its presence at lower levels in other tissues indicates that it participates also in the catabolism of 5Hyl derived from local collagen breakdown.