LMCD1 Monoclonal antibody, PBS Only

LMCD1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A7B3

Cat no : 67834-1-PBS

Synonyms

Dyxin, LMCD1

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Validation Data Gallery

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  WB analysis of U2OS using 67834-1-Ig (same clone as 67834-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67834-1-Ig (LMCD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67834-1-Ig (same clone as 67834-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67834-1-Ig (LMCD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 67834-1-Ig (same clone as 67834-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67834-1-Ig (same clone as 67834-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skeletal muscle using 67834-1-Ig (same clone as 67834-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skeletal muscle using 67834-1-Ig (same clone as 67834-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67834-1-Ig (same clone as 67834-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67834-1-Ig (same clone as 67834-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 67834-1-Ig (same clone as 67834-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 67834-1-Ig (same clone as 67834-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 67834-1-Ig (same clone as 67834-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using LMCD1 antibody (67834-1-Ig, Clone: 2A7B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

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Product Information

67834-1-PBS targets LMCD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LMCD1 fusion protein Ag28331
• Full Name: LIM and cysteine-rich domains 1
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: ~40 kDa
• GenBank Accession Number: BC000646
• Gene Symbol: LMCD1
• Gene ID (NCBI): 29995
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LIM and cysteine-rich domains-1 (LMCD1) is a member of the LIM protein family, which contains an N-terminal cysteine-rich region, two C-terminal LIM domains and a central PET (Prickle, Espinas, and Testin) domain. LMCD1 has been reported in cardiac tissues and lung acting as a transcriptional repressor for GATA6. The mutations of LMCD1 promote cell migration and tumor metastasis in hepatocellular carcinoma. (PMID: 31501411) It has calculated molecular weight around 41kDa.