LIAS Monoclonal antibody

LIAS Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3B5G7

Cat no : 67298-1-Ig

Synonyms

Lip syn, LAS, HUSSY-01, HUSSY 01, EC:2.8.1.8

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Validation Data Gallery

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  WB analysis of LNCaP using 67298-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67298-1-Ig (LIAS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67298-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67298-1-Ig (LIAS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67298-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67298-1-Ig (LIAS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: LNCaP cells, pig brain tissue, HEK-293 cells, K-562 cells, rat brain tissue, mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67298-1-Ig targets LIAS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LIAS fusion protein Ag27286
• Full Name: lipoic acid synthetase
• Calculated Molecular Weight: 372 aa, 42 kDa
• Observed Molecular Weight: 34-42 kDa
• GenBank Accession Number: BC023635
• Gene Symbol: LIAS
• Gene ID (NCBI): 11019
• RRID: AB_2882562
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIAS(lipoyl synthase, mitochondrial) is also named as LAS and belongs to the radical SAM superfamily and lipoyl synthase family. It produces alpha-lipoic acid, an antioxidant and an essential cofactor in alpha-ketoacid dehydrogenase complexes, which participate in glucose oxidation and ATP generation(PMID:22021711). The deduced 373-amino acid protein has a calculated molecular mass of about 42 kD. The N-terminal 26 amino acids encode a potential mitochondrial targeting presequence that, upon removal, would result in a deduced mature protein of 347 amino acids with a molecular mass of about 39 kD(PMID:11389890). Defects in LIAS are a cause of pyruvate dehydrogenase lipoic acid synthetase deficiency (PDHLD).

Publications

Species	Application	Title
human	WB	Sci Rep; Disulfiram downregulates ferredoxin 1 to maintain copper homeostasis and inhibit inflammation in cerebral ischemia/reperfusion injury; Authors - Shuai Yang