LHX8 Polyclonal antibody

LHX8 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30722-1-AP

Synonyms

LIM homeobox protein 8, LIM homeobox 8, Lhx7

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Validation Data Gallery

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  WB analysis using 30722-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30722-1-AP (LHX8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 30722-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using LHX8 antibody (30722-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30722-1-AP targets LHX8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LHX8 fusion protein Ag33787
• Full Name: LIM homeobox 8
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39-44 kDa
• GenBank Accession Number: BC040321
• Gene Symbol: LHX8
• Gene ID (NCBI): 431707
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LHX8 (LIM homeobox 8), also known as LHX7. It is expected to be located in nucleus and mainly expressed in brain, lymphoid tissue, salivary gland. The calculated molecular weight of METAP1 is 39 kDa. The protein encoded by this gene is a member of the LIM homeobox family of proteins, which are involved in patterning and differentiation of various tissue types. These proteins contain two tandemly repeated cysteine-rich double-zinc finger motifs known as LIM domains, in addition to a DNA-binding homeodomain. It is also involved in oogenesis and in neuronal differentiation. LHX8 is a candidate gene for cleft palate, and it is also associated with odontoma formation (PMID: 34112263).