LDHB-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

LDHB-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 19988-1-AP

Synonyms

lactate dehydrogenase B, LDHB, EC:1.1.1.27, LDHA, LDH H

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Validation Data Gallery

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  WB analysis using 19988-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19988-1-AP (LDHB-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19988-1-AP

  WB result of LDHB-Specific antibody (19988-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-LDHB-Specific transfected HeLa cells.

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  IHC staining of human ovary cancer using 19988-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19988-1-AP (LDHB-Specific antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 19988-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LDHB-Specific antibody (19988-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: DU 145 cells, HeLa cells, HEK-293T cells, Jurkat cells, PC-3 cells, mouse heart tissue, rat heart tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

19988-1-AP targets LDHB-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: lactate dehydrogenase B
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_002300
• Gene Symbol: LDHB
• Gene ID (NCBI): 3945
• RRID: AB_10638780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LDHB, also named as NY-REN-46 and LDH-H, belongs to the LDH/MDH superfamily and LDH family. LDHB catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. It is glycolytic enzymes and it is positively regulated by mTOR.Defects in LDHB are a cause of hereditary LDHB deficiency. The antibody is specific to LDHB and has no cross reaction with LDHA and LDHC.

Publications

Species	Application	Title
mouse	WB	Biomedicines; HMTM-Mediated Enhancement of Brain Bioenergetics in a Mouse Tauopathy Model Is Blocked by Chronic Administration of Rivastigmine.; Authors - Renato X Santos
mouse	WB	J Nutr Sci Vitaminol (Tokyo); Combination of Exercise and Intake of Amino Acid Mixture Synergistically Induces Beige Adipocyte Formation in Mice; Authors - Takuya Kojima
human	WB	Mol Biol Rep; Development of a one-plasmid system to replace the endogenous protein with point mutation for post-translational modification studies.; Authors - Iqra Ishrat
mouse	IHC, IF	Am J Physiol Renal Physiol; Expression of lactate dehydrogenase A and B isoforms in the mouse kidney.; Authors - Gunars Osis
human	WB,IF	iScience; Lactate-driven type I collagen deposition facilitates cancer stem cell-like phenotype of head and neck squamous cell carcinoma; Authors - Yunqing Sun ; KD Validated
mouse	WB	iScience; Altered neuronal lactate dehydrogenase A expression affects cognition in a sex- and age-dependent manner; Authors - Ariel K Frame