LDHA Polyclonal antibody

• Featured Product
• KD/KO Validated

LDHA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

51

Reviews (1)

Cat no : 21799-1-AP

Synonyms

LDH-M, LDH-A, LDH A, lactate dehydrogenase A, EC:1.1.1.27

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Validation Data Gallery

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  WB analysis using 21799-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 21799-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 21799-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 21799-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21799-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 21799-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 21799-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 21799-1-AP

  IP result of anti-LDHA (IP:21799-1-AP, 4ug; Detection:21799-1-AP 1:500) with HEK-293 cells lysate 2000ug.

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  IHC staining of human skeletal muscle using 21799-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21799-1-AP (LDHA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 21799-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21799-1-AP (LDHA antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 21799-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LDHA antibody (21799-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 21799-1-AP

  Immunofluorescent analysis of HepG2 cells, using LDHA antibody 21799-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, human testis tissue, A375 cells, HepG2 cells, NIH/3T3 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:30000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 47 publications below
• IHC: See 11 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

21799-1-AP targets LDHA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LDHA fusion protein Ag16703
• Full Name: lactate dehydrogenase A
• Calculated Molecular Weight: 332 aa, 37 kDa
• Observed Molecular Weight: 32-37 kDa
• GenBank Accession Number: BC067223
• Gene Symbol: LDHA
• Gene ID (NCBI): 3939
• RRID: AB_10858925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087).

Publications

Species	Application	Title
mouse	WB	Cell; A tissue injury sensing and repair pathway distinct from host pathogen defense; Authors - Siqi Liu
mouse	IF	Nat Metab; Time of exercise differentially impacts bone growth in mice; Authors - Shaoling Yu
human	WB	Sci Transl Med; Loss of miR-31-5p drives hematopoietic stem cell malignant transformation and restoration eliminates leukemia stem cells in mice.; Authors - Biying Zhu
human	WB,IF,IP	Mol Cell; The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.; Authors - Youming Zhu
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
		Cancer Res; HBXIP and LSD1 Scaffolded by lncRNA Hotair Mediate Transcriptional Activation by c-Myc.; Authors - Yinghui Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Manfred (Verified Customer) (05-30-2022)

This antibody worked perfectly at 1:1000 dilution. I preferred to do overnight incubation at 4 degrees.

• Applications: Western Blot
• Cell Tissue Type: PBMCs