LATS2-Specific Polyclonal antibody

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• KD/KO Validated

LATS2-Specific Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Cat No. 20276-1-AP Copied!

Publications (54)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

LATS2, EC:2.7.11.1, KPM, LATS 2, Serine/threonine-protein kinase kpm

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  WB analysis using 20276-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20276-1-AP (LATS2-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 20276-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20276-1-AP (LATS2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 20276-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20276-1-AP (LATS2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human heart using 20276-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 20276-1-AP (LATS2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 20276-1-AP

  IP result of anti-LATS2-Specific (IP:20276-1-AP, 4ug; Detection:20276-1-AP 1:300) with HEK-293 cells lysate 2320 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse skeletal muscle using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse skeletal muscle using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 20276-1-AP

  Immunofluorescent analysis of HepG2 cells using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human liver cancer tissue, human colon tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20276-1-AP targets LATS2-Specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: LATS, large tumor suppressor, homolog 2 (Drosophila)
• Calculated Molecular Weight: 120 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: NM_014572
• Gene Symbol: LATS2
• Gene ID (NCBI): 26524
• RRID: AB_10697657
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NRM7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LATS2, also named as KPM, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. LATS2 is a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. It negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. It is a negative regulator of the androgen receptor. LATS2 has a calculated molecular mass of 120 kDa and an apparent molecular mass of 150 kDa (PMID: 10871863). The antibody is specific to LATS2.

Publications

What published studies show

The LATS2-Specific Polyclonal antibody (Cat# 20276-1-AP) has 54 citations across journals including Nature Aging, Cell Metabolism, Molecular Cancer, Cell Death & Differentiation, Advanced Science, Science Advances, Oncogene, Cell Reports, and Journal of Biological Chemistry. Research fields span the Hippo/YAP signaling pathway, cancer biology (hepatocellular, gastric, lung, pancreatic, and prostate cancers), ferroptosis, fibrosis, stem cell biology, innate immunity, and aging-related diseases.

Automatically generated from the 54 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cancer; Co-transcriptional R-loops-mediated epigenetic regulation drives growth retardation and docetaxel chemosensitivity enhancement in advanced prostate cancer; Authors - Yufan Ying
mouse	WB	Cell Metab; Cholesterol Stabilizes TAZ in Hepatocytes to Promote Experimental Non-alcoholic Steatohepatitis.; Authors - Xiaobo Wang ; KD Validated
		Nat Aging; Extracellular vesicles derived from senescent hepatocytes drive pan-cancer metastasis in aging; Authors - Huilong Li
human	WB,IHC	J Extracell Vesicles; Extracellular vesicles miR-31-5p promotes pancreatic cancer chemoresistance via regulating LATS2-Hippo pathway and promoting SPARC secretion from pancreatic stellate cells; Authors - Cheng Qin
human, mouse	WB	Autophagy; Mechanical stress-mediated ferritinophagy aggravates cartilage endplate degeneration via a PIEZO1-NCOA4-ZBP1 axis.; Authors - Wenchao Wang
human,mouse	WB	Adv Sci (Weinh); TEAD1 Enhances Exosome Secretion and Promotes Exosome-Mediated Tissue Regeneration.; Authors - Yan Pu