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  • KD/KO Validated

LAPTM4B Polyclonal antibody

LAPTM4B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18895-1-AP

Synonyms

LAPTM4B, LAPTM4beta, LC27



Tested Applications

Positive WB detected inmouse lymph tissue, rat lymph tissue
Positive IHC detected inhuman liver cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18895-1-AP targets LAPTM4B in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LAPTM4B fusion protein Ag13521
Full Name lysosomal protein transmembrane 4 beta
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC014129
Gene Symbol LAPTM4B
Gene ID (NCBI) 55353
RRIDAB_10597385
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LAPTM4B, a novel oncoprotein, is associated with the prognosis of cancer, and may play important roles in the initiation, promotion and metastasis of tumors. It was initially identified as a hepatocellular carcinoma-associated gene, and was recently shown to be up-regulated in various human cancers. Some studies indicated that LAPTM4B may be an useful marker of prognosis in ovarian carcinoma and endometrial cancer. LAPTM4B belongs to the LAPTM4/LAPTM5 transporter family. It has 3 isoforms with molecular masses 41 kDa, 35 kDa and 24 kDa. This antibody can recognize all the 3 isoforms of LAPTM4B.

Protocols

Product Specific Protocols
WB protocol for LAPTM4B antibody 18895-1-APDownload protocol
IHC protocol for LAPTM4B antibody 18895-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IF

Cell

A kinase-independent role for EGF receptor in autophagy initiation.

Authors - Xiaojun Tan
  • KD Validated
WB

Nat Commun

LAPTM4b recruits the LAT1-4F2hc Leu transporter to lysosomes and promotes mTORC1 activation.

Authors - Ruth Milkereit
humanIF

Proc Natl Acad Sci U S A

PtdIns(4,5)P2 signaling regulates ATG14 and autophagy.

Authors - Xiaojun Tan
IHC

Front Cell Dev Biol

Integrative Genome-Wide DNA Methylome and Transcriptome Analysis of Ovaries from Hu Sheep with High and Low Prolific.

Authors - Xiaolei Yao
humanWB,IHC,IF

Biochem Biophys Res Commun

Helicobacter pylori infection promotes epithelial-to-mesenchymal transition of gastric cells by upregulating LAPTM4B.

Authors - Shengfei Zhou
  • KD Validated
humanIF

Cancer Manag Res

LAPTM4B facilitates tumor growth and induces autophagy in hepatocellular carcinoma.

Authors - Fei Wang